海南西海岸真红树内生细菌多样性及其延缓衰老活性研究.pdfVIP

DOI:10.11931/guihaia.gxzw201810032 1海南西海岸真红树内生细菌多样性及其延缓衰老活性研究 李蜜，高程海，姜舒，韦娟，刘思萍，戴恩枝，易湘茜* （广西中医药大学海洋药物研究院/药学院/广西中药药效研究重点实验室，南宁 530200 ） 摘要：设计9 种分离培养基，采用稀释涂布法从14 份真红树植物的46 份组织样品中分离纯化内生细菌。 基于菌株形态学特征和 16S rRNA 基因序列确定分离菌株的种属，并分析其物种多样性，采用秀丽隐杆线 虫模型筛选菌株延缓衰老活性。结果表明：通过基因序列去重复后从 46 份真红树植物组织样品中获得 32 株海洋细菌，基于菌株16S rRNA 基因序列信息分析，覆盖 12 科17 属，其中芽孢杆菌属（Bacillus ）为优 势菌属，并获得1 株疑似橙单胞菌属（Aurantimonas ）新种，16S rRNA 基因序列相似性低于97% 。经过秀 丽隐杆线虫粗筛发现3 株海洋细菌具有显著延缓线虫衰老的活性 （P0.05 ）。研究结果表明海南西海岸真红 树内生细菌具有物种多样性，部分菌株具有延缓线虫衰老活性。 关键词：真红树植物，内生细菌，多样性，延缓衰老活性 中图分类号：R946 ，Q939.1 文献标识码：A Study on diversity and anti-aging activity of endophytic bacteria from true mangrove plants collected from the west coast of Hainan * LI Mi, GAO Chenghai, JIANG Shu, WEI Juan, LIU Siping, DAI Enzhi, YI Xiangxi （Institutes of Marine Drugs/Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica/Faculty of Pharmacy , Guangxi University of Chinese Medicine, Nanning 530200, China ） Abstract: The bacteria were isolated and purified from forty-six tissues fourteen true mangrove plants used nine kinds of isolation medium by dilution coating method. The strains diversity and genera were analyzed by bacteria morphological characteristics and 16S rRNA gene sequences. The Anti-aging activities of the bacteria were tested by Caenorhabditis elegans screening models. The results showed that 32 marine bacteria were obtained by the 46 true mangrove plants tissue. The 32 bacteria to comparison of 16S rRNA gene sequences which could be classified into 7 families and 11 genera. The dominant genus were Bacillus . The 16S rRNA gene sequence similarities between 1 strains and the related species were all less than 97% have been already proved to be novel species of the genera Aur