低温胁迫对葡萄幼苗相关生理指标的影响.docVIP

低温胁迫对葡萄幼苗相关生理指标的影响 安徽农业科学,JournalofAnhuiA.Sci.2OO8.36(4):1351—1352.1364责任编辑庆珞责任校对马君叶 低温胁迫对葡萄幼苗相关生理指标的影响 曹慧,赵升,王孝威,崔英(潍坊学院生物系,山东潍坊26l061) 摘要[目的]探究低温胁迫对葡萄幼苗相关生理指标的影响.[方法]选用早黑宝葡萄试管幼苗为材料,当长至5～6片真叶时挑选长 势相同的葡萄幼苗进行常温25(CK),15,5℃3种不同温度处理,于处理后0…246,8d进行各项生理指标测定,之后恢复常温处理,即恢 复2d(R2),4d(R4),测定相关生理指标,研究不同程度低温处理对葡萄试管幼苗形态指标和生理指标(叶绿素含量,可溶性糖含量,SOD 活性,POD活性,MDA活性及质膜相对透性(PMP))的影响.[结果]葡萄试管幼苗经低温胁迫后,叶绿素含量下降,可溶性糖含量升高; SOD,POD活性先升后降;低温造成葡萄试管苗的膜脂过氧化,MDA含量升高,P增大.[结论]15℃低温处理对葡萄试管幼苗的伤害 是可逆的,而5℃处理造成了不可逆伤害. 关键词葡萄;低温胁迫;生理指标 中图分类号Q945.78文献标识码A文章编号0517—6611(2oo8)04—01351—02 EffectsofLowTemperatureStressOntheRelatedPhysiologicalIndexesinGrapeSeedUl~ CAt)删etal(DepartmentofBiology,WeifangUniversity,Weifang,Shandong261061) Abstract[Objective]rn1eresearchaimedtoexploretheeffectsoflowtemperatureS[1~SSontherelatedphysiologicalindexesingrapese~ings. 【Method]UsingtheseedlingsofZaoheibaograpeinvitroasmaterials,thegrapeseedlingswith5～6euphyllaandthesalTlegrowthvigorweretreatedwith 3kindsoftern~mtureincludingnormaltemperature(25℃asCK),15℃and5℃.AⅡthephysiologicalindexesweredeterminedon0th,2nd,4th, 6th,8thafterthetreatments.Andthenthetreatmentofnormaltemperaturewasresumedfor2d(R2)and4d(R4)todetemainetherelatedphysiologi— calindexes.nleeffectsofdifferentdegreeoflowtemperaturetreatmentsonthemorphologicalindexesandthephysiologicalindexes(chlorophyllcontent. thecontentofsolublesugar,SODactivity,P0Dactivity,MDAactivityandrelativeperneabilityofplasmamembrane(PMP))ofgrapeseedlingsinvitro werestudied.[Result]AfterlowtemperaturesⅡ℃ss,chlorophyllcontentofgrapeseedlingsinvitrowasdecreasedandthecontentofsolublesugarwasin— creased,andtheactivitiesofSODandPODwerefirstincreasedandthendecreased.Lowtemperaturecausedthepemxidationofmembranelipidingrape s~mgsinvitro,theincreaseofMI】AcontentandtheenlargementofP.【ConclusionJnleinjur)『oflowtemperaturetreatmentat15℃togrape seedlingsinvitroWasreversibleandthetreatmentat5℃causedtheirreversibleinjury. KeywordsGrape;Lowtemperatureslress;Physiologicalindex 葡萄(VitisvineferaL.)设施栽培可克服其不耐贮运,