牵引成骨过程中生理性负重对新生血管影响地实验研究.docxVIP

A study of the influence of Physiologic Weight-bearing for New Vessel Formation during Distraction Osteogenesis Postgraduate：Wang yuanshun Supervisor：Prof．Tang xin Maj or subj ect：orthopedics Abstraet Distraction o steogenesis(DO)can generate new bone tissue in the bone crevice or between the two sides of broken bone by slow，incremental distraction of fracture callus in a correct pull strength to stimulate and prolong active new bone formation，which is a process for lengthening long bones or filling segmental bone defects．DO is a new therapy technology which has lors of advantages which can’t be reached by tradition method． The key of DO is that highly qualitative bone is generated，which is closely linked to whether there is enough blood supply or not．Many studies suggested that the new bone formation was usually accompanied by angiogenesis．So it is very important for new vessel formation to shorten the therapy period，decrease new bone dysplasia，and encourage the clinical application of DO． Objective：To observe the influence of physiologic weight bearing(WB) and non—weight—bearing for new vessel formation in the new bone formation area in rabbit femoral lengthening models during distraction osteogenesis． Methods：Unilateral right femoral lengthening were performed in 1 8 male N—W rabbits(3．5-4．0kg，Age：6m一12m)which were divided into two groups randomly．One group(A)were allowed to bear weight freely(WB) while the other(B)were made non—weight-bearing by a through—knee amputation(NWB)．After a seven—day latency period，femurs were lengthened 0．35mm twice a day．and 14．7mm over 21 days．Animal s were sacrificed at 7，21，35，and 49 days(0，9．8，14．7，and 14．7mm of distraction)． The bony specimens were collected and prepared(fixation，decalcification， dehydration，paraffin imbedding，section) for immunohistochemical of specific antibodies to endothelial cell antigen(CD34)observation and 3 semi—quantitative examination of microvessel by Chalkley counting method， then the results was