初探瑞舒伐他汀对大鼠颈动脉新生内膜增生的影响及机制（可编辑）.docVIP

初探瑞舒伐他汀对大鼠颈动脉新生内膜增生的影响及机制 暨南大学硕士学位论文 初探瑞舒伐他汀对大鼠颈动脉新生内膜增生的影响及机制 中文摘要 目的:初步探讨瑞舒伐他汀对大鼠颈动脉球囊损伤后新生内膜增生的影响及其作用机制。 方法:48 只雄性 SD 大鼠随机分为对照组、损伤组和治疗组,每组 24 只(对照组与损伤 组共用 24只大鼠)。 48只大鼠均建立左颈总动脉球囊损伤模型,并取损伤组右颈总动脉(未 行球囊损伤)作为对照组。治疗组于术前 3 d始连续每 d予瑞舒伐他汀 5 mg/kg?d灌胃, 损伤组予生理盐水灌胃。术后 7 d和 14 d取颈总动脉进行 HE染色,TUENL 法检测细胞凋 亡,免疫组化法检测 SM α-actin、PCNA、PTEN和磷酸化 PTENp-PTEN,并用蛋白印迹 法定量检测 PTEN、p-PTEN的表达。 结果:1 治疗组 14 d,血管新生内膜、新生内膜/中膜面积比值较损伤组 14 d明显减少, 管腔面积增加 26%P?0.05。2 治疗组 14 d,PCNA 阳性细胞率为15.6±2.1%,凋亡阳 性细胞率为26.8±2.7%,与损伤组 14 d 比较,差异有统计学意义P?0.05。3 PTEN、 p-PTEN 主要表达于新生内膜血管平滑肌细胞VSMCs胞浆。4 与损伤组比较,治疗组 7 d 、14 d p-PTEN/总 PTEN比值均显著减少P?0.05)。 结论:1 瑞舒伐他汀对大鼠颈动脉新生内膜的增生和血管再狭窄有抑制作用。2 瑞舒 伐他汀可促进 VSMCs 凋亡并抑制 VSMCs 增殖。3 瑞舒伐他汀可降低损伤动脉 p-PTEN/ 总 PTEN比值。 4 瑞舒伐他汀可通过促进 VSMCs凋亡并抑制其增殖而减少新生内膜形成、 抑制血管再狭窄,该作用可能与其促进 PTEN 的表达并抑制 PTEN的失活有关。 关键词:球囊损伤; 新生内膜增生; 血管平滑肌细胞; 瑞舒伐他汀; PTEN I暨南大学硕士学位论文 初探瑞舒伐他汀对大鼠颈动脉新生内膜增生的影响及机制 ABSTRACT Objective: To preliminarily investigate the influence of Rosuvastatin on the neointimal hyperplasia and its mechanism in rat carotid arteries after balloon injuryMethods: 48 male SD rats were randomly and equally divided into Rosuvastatin group, the injured group and control group, and the latter two groups shared 24 rats. Each rat was subjected to balloon injury on the left common carotid artery, and control artery without injury was on the right artery. Rosuvastatin group rats were given Rosuvastatin dissolved in Nacl, gavage at 5 mg /kg?d 3 days before injury until sacrificed, while the injured group rats were given vehicle instead .All rats were sacrificed on 7 d or 14 d after injury, and the common carotid arteries were harvested for HE staining and TUNEL apoptosis analysis. Meanwhile, SM α-actin, PCNA, PTEN and phosphor-PTEN p-PTEN were detected by immunohistochemistry, and the changes of PTEN and p-PTEN expression were measured by Western-blotResults: 1 The area of neointimal and the area ratio of neointimal to media decreased significantly in 14 d in Rosuvastatin gr