ATP通过激活AktmTORSTA罗T3信号转导通路促进脊髓损伤修复.docxVIP

优秀毕业论文 精品参考文献资料 兰州大学硕托研究生学位论文C 兰州大学硕托研究生学位论文 C组，低于D组，差异有统计学意义(P0．05)。免疫组织化学、 Western．blot、实时荧光定量PCR检测均显示，术后1--4周，A组大鼠 Akt、mTOR、STAT3、NSE表达持续增高， Nestin表达逐渐降低， 均高于B、C、D组，差异有统计学意义(PO．05)；而A组 GFAP表达持续增高，均低于B、C组，高于D组，差异有统计学意义 (P0．05)。各时间点B、C组各指标表达与D组比较差异均有统计 学意义(PO．05)，B、C组间Western．blot检测的Akt、P．Akt、mTOR、 P．mTOR、STAT3、P．STAT3比较有差异，余指标表达差异无统计学意 义(P0．05)。 结论 1．ATP治疗促进大鼠SCI修复 2．ATP在SCI大鼠体内可激活Akt／mTOR／S丑玎3信号系统 3．ATP活化的Akt／mTo刚S仉叮3通路能诱导内源性NSCs增殖分化 为神经元 4．在大鼠体内ATP能激活Akt／mTOR／STAT3通路诱导内源性 NSCs增殖、分化为神经元，有助于SCI的愈合。 【关键词】 丝氨酸／苏氨酸蛋白激酶哺乳类动物雷帕霉素靶 蛋白 信号转录和转导激活因子3三磷酸腺苷脊髓损伤信号通 路 兰州人学硕士研究生学位论文ATP 兰州人学硕士研究生学位论文 ATP promotes the repair of spinal cord inj ury by activating the Akt／mTOR／STAT3 signal transduction pathway in rats Abstract Objective Endogenic neural stem cells(NSCs)can differentiate into neurons leading to me healing of SCI，however，its molecular mechanism is not clear in SCI．In this study，we aimed to investigate the mechanism of ATP·-activated serine／threonine··specific protein kinase (Akt)／mammalian target of rapamycin(mTOR)／signal transducer and activator of transcription 3(STAT3)pathway in the physiology and pathology of spinal cord injury(SCI)． Methods Adult specific pathogen·-free female Sprague-·Dawley rats (128)were randomly divided into four groups 0=32)：Using a modified Allen’S stall to produce model of SCI at T8。10 in rats of group A B，C． Group A in which group rats were subjected to the administration of ATP，for 7 days；Group B，in which group rats were subjected to the administration of physiological saline(equal·volume)，for 7 days；Group C，in which group rats were subjected to the administration of ATP and rapamycin，for 7 day；Group D，in which group rats were subjected to laminectomy in combination with the administration of physiological saline(equal-volume)，for 7 days．Locomotor activitys of rats were evaluated using the Basso，Beattie，and Bresnahan(BBB)rating scale at ．．III． 兰州大学硕十研究生学位论文postoperative 兰州大学硕十研究生学位论文 postoperative 1，2，3，4 weeks．Then，detecti