斑马鱼对无氧运动训练的适应性变化.pdf

动 物 学 研 究 2013，Jun. 34(3): 190−195 CN 53-1040/Q ISSN 0254-5853 Zoological Research DOI:10.11813/j.issn.0254-5853.2013.3.0190 斑马鱼对无氧运动训练的适应性变化 1 2,* 刘明镜 ，王志坚 1. 重庆医科大学 附属儿童医院，认知发育与学习记忆障碍转化医学重庆市市级重点实验室，重庆 400014 ； 2. 西南大学 淡水鱼类资源与生殖发育教育部重点实验室，水产科学重庆市市级重点实验室，生命科学学院，重庆 400715 摘要：该研究以斑马鱼为 (Danio rerio)对象，研究了四周无氧运动训练对斑马鱼行为、形态、生长、肌肉生化组分及代谢酶 活性的影响。旨在探索斑马鱼对无氧运动训练的适应性变化，为进一步了解鱼类适应无氧运动训练的分子机制提供基础数据。 结果发现：斑马鱼的日常活跃程度经四周无氧运动训练后显著降低，群聚程度增加；训练组个体体重和体长增长减缓，更利 于运动；肌糖原含量显著增加，运动持久能力加强；肌肉乳酸脱氢酶 (LDH) 活性显著增高，柠檬酸合成酶 (CS) 活性显著 降低，无氧代谢能力加强。即，斑马鱼无氧运动能力和无氧代谢能力在训练后得以明显提升。 关键词：无氧运动训练；斑马鱼；形态；酶；适应 中图分类号：Q955 ；Q959.468 文献标志码：A 文章编号：0254-5853-(2013)03-0190-06 Adaptive changes of Zebrafish (Danio rerio) to anaerobic exercise training Ming-jing LIU 1, Zhi-jian WANG 2,* 1. Chongqing Key Laboratory of Translational Medical Research in Cognitive Development and Learning and Memory Disorders, Children ’s Hospital of Chongqing Medical University, Chongqing 400014, China; 2. Key Laboratory of Fresh Water Fish Reproduction and Development, Education of Ministry, Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University, Chongqing 400715, China Abstract: To explore adaptive changes of the Zebrafish (Danio rerio) to anaerobic exercise training as well as to collect basic data of molecular mechanisms of adaption to anaerobic exercise training among this fish, we investigated the influences of 4 weeks of anaerobic exercise training on the behavior, morphology, growth, muscle biochemical components and metabolic enzyme activities of the Zebrafish. Our results indicated that individual’s daily activity level declined after 4 weeks training and they preferred to swim together m