幽门螺杆菌粪便抗原检测.pptxVIP

幽门螺杆菌粪便抗原检测; ;第四次全国幽门螺杆菌感染处理共识报告;对于无示警症状者的test and treat策略;Tests for Helicobacter pylori infection;常用检测Hp方法对比总结;Title: Evaluation of enzyme-linked immunosorbent assay for the diagnosis of Helicobacter pylori infection in children from different age groups with and without duodenal ulcer. Author: de Oliveira, A. M.; Rocha, G. A.; Queiroz, D. M. (...) Source: J Pediatr Gastroenterol Nutr, 1999, 28(2): 157-161 Abstract: BACKGROUND: ... METHODS: A second-generation ELISA was used to evaluate the IgG response to H. pylori in the serum of 130 consecutive children who underwent upper gastrointestinal endoscopy. The presence of H. pylori was determined in antral biopsy specimens by culture, urease test, and histologic analysis. RESULTS: Sixty-eight children (all of the 20 who had duodenal ulcer) were H. pylori positive by microbiologic test. Immunoglobulin G antibodies to H. pylori were detected in 79.4% of the infected children and in 8.1% of the noninfected ones. The sensitivity of the test was higher in patients with duodenal ulcer (100%) than in those without (70.8%). When used in children of different ages the test also presented differences in sensitivity: 44.4% in children 2 to 6 years old; 76.7% in children 7 to 11 years old, and 93.1% in children 12 to 16 years old (p = 0.006). The serum immunoglobulin G concentration was significantly higher (p = 0.0003) in children with duodenal ulcer than in those without and was higher in older children than in younger ones without duodenal ulcer (p = 0.05). CONCLUSIONS: The accuracy of the test in children with duodenal ulcer and in children more than 12 years old was good; however, in children up to 12 years of age without duodenal ulcer, the sensitivity of the test was too low to be used for screening purposes or to rule out the presence of infection.;1.Non-invasive serological tests have been widely used for the diagnosis of H. pylori infection. In adults, this method has proved to be highly accurate to dia