蒽酮-硫酸法与苯酚-硫酸法测定凉粉草多糖的比较.docVIP

蒽酮-硫酸法与苯酚-硫酸法测定凉粉草多糖的比较 王文洁1,2，唐炜1,2，俞玲娜1,2，基金项目：国家自然科学基金项目；江西省自然科学基金青年重大项目（ 基金项目：国家自然科学基金项目；江西省自然科学基金青年重大项目（20152ACB21004） 作者简介：王文洁 (1992-)，女，硕士研究生，研究方向：食品营养。 E-mail:Wangwenjie999777@163.com *通信作者：谢建华(1982-)，男，研究员，博士，研究方向：食品化学与营养。Email: jhxie@ (1. 南昌大学 食品科学与技术国家重点实验室，南昌330047；2．南昌大学 食品学院，南昌330047) 摘要：比较蒽酮-硫酸法与苯酚-硫酸法测定凉粉草多糖含量的差异，以期选择较好的方法对凉粉草多糖进行测定。结果表明：苯酚-硫酸法最佳测定条件为苯酚溶液5%、浓硫酸5.0 mL、反应温度100 °C、显色时间30 min，测得凉粉草中含糖量为22.06%。蒽酮-硫酸法最佳测定条件为硫酸浓度98%，反应温度80 °C、反应时间20 min、取浓度为2.0 g/L蒽酮-硫酸液的体积3.0 mL，测得凉粉草中含糖量为27.79%。较苯酚-硫酸法精密度和重现性RSD 1.31%，稳定性RSD 0.22%，加标回收率均不小于115%，蒽酮-硫酸法的精密度和重现性RSD 1.00%，稳定性RSD 0.11%，加标回收率均不小于102.1%。结论：两种方法均操作简便,准确可靠、稳定可行。 关键词：苯酚-硫酸法，蒽酮-硫酸法，凉粉草多糖，测定 Comparison of Anthrone-Sulfuric and Phenol-Sulfuric Acid Methods for Determination of Polysaccharide in Mesona Blume WANG Wen-jie1,2, TANG Wei1,2, YU Ling-na1,2, XIE Jian-hua1,2,*, XIE Ming-yong1,2 (1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China； 2. School of Food Science and Technology, Nanchang University, Nanchang 330047, China) Abstract: To compare the difference in determination content between phenol-vitriolic method and anthrone-sulphuric acid method for polysaccharides in Mesona Blume. The results showed that the optimum conditions for the determination of phenol-vitriolic method were 5% aqueous phenol solution, 5 mL of concentrated sulfuric acid, reaction temperature 100 °C and reaction time 30 min, the content of sugar was 22.06%. The results showed that the optimum conditions for the determination of anthrone-sulphuric acid method were 98% of concentrated sulfuric acid reaction temperature 80 °C and reaction time 20 min and 3mL anthrone-sulphuric acid, the content of sugar was 27.79%. Through the methodology comparison, The purity and reproducibility of phenol-vitriolic method were 1.31%, the stability was 0.22%, the recoveries were less than 115%, the precision and reproducibility of anthrone-sulphuric acid method were 1.00%, th