一株产蛇孢假单壳素真菌F02Z2172的形态和分子鉴定-微生物学报.pdf

微生物学通报 AUG 20, 2010, 37(8): 1205−1210 Microbiology China © 2010 by Institute of Microbiology, CAS tongbao@ 简 报 一株产蛇孢假单壳素真菌 F02Z2172 的形态 和分子鉴定 * 李业英 石英 路新华 崔晓兰 马瑛 赵颖 刘静 张华 (华北制药集团新药研究开发有限责任公司 微生物药物国家工程研究中心 河北省工业微生物代谢工程技术 研究中心 河北 石家庄 050015) 摘 要: 从厦门武夷山自然保护区土壤样品中分离得到一株可产生蛇孢假单壳素(Ophiobolins) 的 丝状真菌, 编号为F02Z2172 。通过对其宏观特征、显微特征进行观察并对其ITS 、Calmodulin 和 β-tubulin 序列进行分子系统学分析, 鉴定为伪弯头曲霉, 并对伪弯头曲霉与焦色组内其他菌种的 关系进行了讨论。由伪弯头曲霉产生蛇孢假单壳素还是首次报道。 关键词: 蛇孢假单壳素, 伪弯头曲霉, 转录间隔区, 钙调素基因, β-微管蛋白基因, 焦色组 Morphological and Molecular Identification of Ophiobolins Producing Strain F02Z2172 * LI Ye-Ying SHI Ying LU Xin-Hua CUI Xiao-Lan MA Ying ZHAO Ying LIU Jing ZHANG Hua (NCPC New Drug Research and Development Co.,Ltd. , National Engineering Research Center of Microbial, Medicine Hebei Industry Microbial Metabolic Engineering Technology Research Center , Shijiazhuang, Hebei 050015, China) Abstract: A filamentous fungus F02Z2172 which is able to produce ophiobolins was isolated from Wuyishan Nature Reserve in Fujian province. The strain was identified as Aspergillus pseudodeflectus by cultural and morphological characteristics and phylogenetic analysis based on ITS rDNA, Calmodulin and β-tubulin sequences. The relation between A. pseudodeflectus and other species of As- pe