山西省番茄早疫病菌对苯醚甲环唑的敏感性及抗性突变体的适.PDFVIP

植物保护学报Journal of Plant Protection ，2019 ，46 （1）：201-207 DOI ：10.13802/ki.zwbhxb.2019.2017174 山西省番茄早疫病菌对苯醚甲环唑的敏感性 及抗性突变体的适合度 1* 2 1 1 史晓晶 任 璐 王华杰 韩佳音 （1. 忻州师范学院生物系，忻州034000 ；2. 山西农业大学农学院，太谷030801 ） 摘要：为了解山西省番茄早疫病菌Alternaria solani 对苯醚甲环唑的抗性，采用菌丝生长速率法检 测了该病菌对苯醚甲环唑的敏感性，分析了抗性水平和抗性指数；同时通过室内诱导获得了苯醚甲 环唑抗性突变体，检测了突变体的抗性遗传稳定性、交互抗性和适合度。结果表明：分离自大同、忻 州、长治、晋中和运城5 市的101株菌株的EC50 范围为0.005~3.315 mg/L ，均值为0.428 mg/L ，其中敏 感菌株占97.03%，低抗菌株占2.97%；抗性指数范围为0.295~0.447 ，均值为0.343。10株中抗突变 体抗性均不能稳定遗传、生长速率减慢、产孢量降低、孢子萌发能力减弱、致病力下降；但在药剂压 力下，突变体的致病力强于敏感菌株。苯醚甲环唑与代森锰锌、多抗霉素、啶酰菌胺无交互抗性，而 与啶菌噁唑存在交互抗性。因此，今后需加强番茄早疫病菌对苯醚甲环唑的田间抗性监测，在生产 过程中轮用或混用不同作用机制的药剂以延缓该病菌对苯醚甲环唑抗性的发展。 关键词：番茄早疫病菌；苯醚甲环唑；敏感性；适合度 Sensitivity of early blight pathogen Alternaria solani to difenoconazole and fitness of resistant mutants Shi Xiaojing1* Ren Lu2 Wang Huajie1 Han Jiayin1 （1. Department of Biology, Xinzhou Teachers University, Xinzhou 034000, Shanxi Province, China; 2. College of Agriculture, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China） Abstract: To understand the resistant situation of early blight pathogen Alternaria solani to difenocon- azole in Shanxi Province, the sensitivity to difenoconazole were tested, and the resistance level and the resistance index were analyzed. Also, the difenoconazole-resistant mutants, which were induced in the laboratory, were studied on the genetic stability of resistance, the cross-resistance and the fitness. The re- sults showed that the EC50 values of 101 isolates from Datong, Xinzhou, Changzhi, Jinzhong, and Yuncheng cities ranged from 0.005 to 3.315 mg/L with the mean value of 0.428