红鲤4群体间主要组织相容性复合体的差异.pdf

272 Vol.27, No. 2 2003 4 JOURNAL OF FISHERIES OF CHINA Apr., 2003 : 1000- 0615(2003)02- 0113- 06 4 蔡完其, 轩兴荣, 王成辉, 邹曙明 ( , 200090) :( MHC), MHC , , NA MHC ,,( 1) MHC 2 2 ,234bp 106 ,45.3%; 3; (2) MHC , 2 ,, , ;(3)MHC, 2 :; ;; ;; + :S917;Q343. 1 7 :A The variations of major histocompatibility complex in four populations of red common carp CAI Wanqi, XUAN Xingrong, WANG Chenghui, ZOU Shuming (Key Laboratory of A quatic Genetic Resources andA quacultural Ecology Certfi icated by the Ministry of Agriculture, Shanghai Fisheries Uni ersity , Shanghai 200090, China) Abstact:The major histocompatibility complex (MHC) of fish was used to study the genetic structure as a molecular genetic marker of four populations of red common carp. Based on reported common carp MHC class gene sequences, a pair of specific primers was designed, MHC class molecular 2 gene fragments were amplified from Xingguo red common carp ( Cyp rinus carp io var. singguonensis ) , purse red common carp ( C. carp io var. wuyuanensis ) , glass red common carp ( C. carp io var. wananensis) , and Oujiang color common carp ( C. carp io var. color) , then cloned and sequenced. Thepolymorphism of encoded MHC classmolecular chain gene was rich, with 106 variable loci in 234bp in length. The percentage of polymorphism was 45.3%, 2 among the four populations, pursered common carp was significantly different from the other red common carps. The phylogenetic trees constructed by encoded MHC classmolecular chain gene and its amino acid