稿件修改说明与对专家审稿意见答复.doc

稿件修改说明与对专家审稿意见答复 稿件题目：磺酸咔咯及其镓（Ⅲ）配合物与DNA的相互作用和光核酸酶活性 稿件作者：黄俊腾，张 阳，王湘利，计亮年，刘海洋 稿件编号：130035 《无机化学学报》编辑部： 根据审稿专家的建议，我们对本文进行了认真修改，还补充了少量实验数据。现就该文的修改说明与对审稿专家提出的问题答复如下： 一、第一审稿人评审意见： 1. Characterization of the complex 1-Ga. 1-Ga represents a new complex. This reviewer recommends the author to consider whether it is useful to report the crystal structure (if possible) in combination with elemental analysis. Is m/Z = 1021.932 in the mass spec the only peak observed? Isn’t [M–2Na? 2H ] a neutral species as opposed to the singly charged negative species? The NMR data looks reasonable to this reviewer; however, the authors should consider showing a drawing of the ligand in a scheme to present peak assignments for the NMR spectra. In summary, proper characterization of the complexes that demonstrate purity in bulk is essential for inorganic studies generally speaking. So this reviewer would really like to see improvement in this regard for this manuscript.答复： 2001年，Gross课题组报道了水溶性咔咯1的合成[Org. Lett., 2001, 3(22):3443-3446]，随后报道了其镓配合物1-Ga的晶体结构图[J. Am. Chem. Soc., 2002, 124(25):7411-7420]。目前1和1-Ga的光核酸酶活性尚未见报导，本文着重研究其光核酸酶活性。由于最近我们刚发表了1-Mn的文章[本文引用文献28：Transition Met Chem., 2013, 38(3):283-289]，在该文章中已经给出1的表征数据。故在本稿件中只给出1-Ga的表征数据。 在1-Ga的质谱图中（MALDI-TOF）主要有两个峰，m/z=1021.932是质谱的主峰（100%），由于质谱采用的是-MS模式，故将质谱峰归属为[M-2Na+2H]-；而m/z=1057.936则归属为[M-2Na+2H+2H2O]- （27%）。质谱数据与1-Ga理论分子量一致。为了避免混淆，正文质谱数据“MALDI-TOF，negative” 修改为“MALDI-TOF，negative ion detection mode”。并补充了水合峰数据[M-2Na+2H+2H2O]- （27%） 稿件中列出1-Ga的NMR数据与文献同。为了方便读者更好了解1H-NMR的归属，修改稿已经重新画过Scheme 1，标明了各个氢原子的位置。 同时在表征数据中表明H的归属位置: 1H NMR (400 MHz, CD3OD) δ = 9.91 (s, Ha), 8.85 (s, Hb), 8.79 (d, J = 4.5 Hz, Hc), 8.66 (d, J = 4.6 Hz, Hf), 8.54 - 8.61 (m, Hd,e); 咔咯类大环化合物常用的表征手段是Uv-vis、MS和NMR，本文相应的表征数据与文献报道相同。 Scheme 1 2. The UV-vis section is overall well presented. However, there are a few things that need to be addressed. For Figure 3, the fonts of the inset are too small for this reviewer to read. What is the y axis of the inset in Figure 3? If it i