硫化氢对大鼠离体心脏低温保存效果的实验研究 医学论文.docVIP

???? 硫化氢对大鼠离体心脏低温保存效果的实验研究 医学论文 ???? ???? ????　　本文由中国论文范文收集整理。 ??????????????????????作者：白崇峰，崔勤，李亮，李彤? 【摘要】? 目的 观察硫化氢（H2S）对大鼠离体心脏的保护作用。 方法 将48 只SD大鼠随机分成对照组（ST组）和实验组（HST组），每组24只，分别采用St.Thomas Ⅱ液和St.Thomas Ⅱ液+H2S为停搏液及保存液，保存心脏4 h，6 h，8 h。采用Langendorff心脏灌注和工作模型装置进行心脏保存前及保存后再灌注30 min心功能测定,比较心功能恢复率、心肌含水量、心肌酶［乳酸脱氢酶（LDH）及磷酸肌酸激酶（CK）］漏出量以及心肌组织超微结构变化。结果 保存4 h、6 h后，HST组心功能指标及心肌含水量与ST组差别无统计学意义，但心肌酶漏出量明显减少；保存8 h后HST组所测各指标均优于ST组。结论 加入H2S的St.Thomas Ⅱ液可以提高大鼠离体心脏的保存效果。 【关键词】? 硫化氢；心脏移植；器官保存；大鼠 　　Abstract: OBJECTIVE?? To observe the protective effects of hydrogen sulfide on isolated rat hearts. METHODS? Forty-eight SD rats were randomly assigned to two groups : control group(ST group，n=24) , the hearts were arrested with St.Thomas Ⅱ solution and preserved in the same solution;study group（ HST group，n=24）,the hearts were arrested with St.Thomas Ⅱ plus hydrogen sulfide (H2S) solution and preserved in the same solution. All rat hearts were preserved for 4 h, 6 h and 8 h with 4℃ hypothermia, and underwent 30 min reperfusion (37 ℃). The isolated hearts were perfused on the Langendorff perfusion apparatus for functional evaluation before and after preservation. Myocardial enzyme? leakage，wet weights in myocardium and cardiac ultrastructure were measured after reperfusion. RESULTS? After the hearts were preserved for 4 h and 6 h, the differences of the recovery of myocardial function and wet weights in myocardium were insignificant，but the differences of creatine kinase(CK) and lactic dehydrogenase (LDH) were significant between HST and ST group. After the hearts were preserved for 8 h, the differences of all target measured were significant between HST and ST group.CONCLUSION? Addition of H2S into the St.Thomas Ⅱ solution could enhance the myocardial protective effects to the isolated rat hearts. 　　Key words：? H2S；Heart transplantation；Organ preservation; Rat 　　随着国内外心脏移植的 发展 ,供心不足及供心保存时限短严重制约了其进一步发展。迄今为止, 供心的冷保存时限仍被限制在4～ 6 h 以内［1］。加强供心保护，提高供心保存时限和保存质量，对移植手术的成败具有重要意义。??? 　　近年来实验证明，内源性H