烟草和拟南芥PAPK对部分NCAPs的识别.ppt

Page ? * ——TMV MP磷酸化促进大分子通过胞间连丝而转移 烟草和拟南芥PAPK对部分NCAPs的识别 CONCEPTS Plasmodesma PAPK NCAPs (TMV MP为例) Plasmodesma 胞间连丝见于所有的高等植物、某些低等植物如有些藻类以及真菌。 在有胞间连丝的植物中，多至平均每平方微米140 个。 如高等植物顶端分生组织的胞间连丝分布状况就与分化的控制有关 例如蕨类植物桂皮紫萁胞间连丝在垂周壁与平周壁的分布数目不同就影响了顶端早期叶的发育。 初生胞间连丝是高尔基器小泡融合成细胞板时，因被伸入于其间的内质网膜阻止其融合而形成的。 次生胞间连丝是由一些降解酶（果胶酶、半纤维素酶和纤维素酶）的作用使完整的细胞壁穿孔而成。 Structure of plasmodesma PAPK Plasmodesmal-Associated Protein Kinase 34-kD calcium-dependent protein kinase activity the phosphorylation of TMV MP, by PAPK, was calcium independent Interestingly, both GTP and ATP could be used as good phosphate donors for the tobacco PAPK Peptide Microsequence Analysis Identifies PAPK as a Member of the CKI Gene Family NCAPs In plants, cell-to-cell communication is mediated by plasmodesmata and involves the trafficking of non–cell-autonomous proteins (NCAPs). Plants in which the NtNCAPP1 gene was silenced were compromised in their ability to regulate leaf and floral development, supporting a model in which NCAP delivery to plasmodesmata is both selective and regulated. PROCESS PAPK1 Colocalizes with TMV MP step1 step2 step3 PAPK Phosphorylates TMV MP at Its C Terminus conformation of NCAP changed, making aperture of plasmodesmata enlarged PROCESS GFP标记的亚细胞定位观察发现，CK1家族的蛋白（如PAPK）多定位于韧皮部细胞的胞壁两侧 病毒的及植物细胞中的部分NCAPs（如TMV MP）被共定位的PAPK识别，并C端磷酸化 病毒进入植物细胞后复制，产生运动蛋白，运动蛋白与病毒RNA结合，形成无折叠的转移复合体； 运动蛋白引导复合体到胞间连丝处； 被PAPK识别，并C端磷酸化的运动蛋白引导胞间连丝孔径扩大； 复合体通过胞间连丝或解离的核酸通过胞间连丝而运动蛋白留在胞间连丝上。 其它的一些蛋白质也可能与胞间连丝结构蛋白相互作用，使胞间连丝的通透性能上升并使得大分子蛋白质胞间转移。 PROCESS Thank you 参考文献:Lee et al., 2005, Plasmodesmal-Associated Protein Kinase in Tobacco and Arabidopsis Recognizes a Subset of Non-Cell-Autonomous Proteins. ThePlantCell 17:2817-2831. Lee et al.,2003, Selective Trafficking of Non-Cell-Aut