年产110吨肝素酶发酵车间工艺设计.pdf

年产 110 吨肝素酶发酵车间工艺设计 摘要 肝素酶指一类能够特异性裂解肝素和类肝素主链糖苷键的酶,被用于清除血 液中的肝素,测定肝素的结构, 以及抗肿瘤等医学方面的研究。国外对肝素酶的研 究已有 40 多年历史,涵盖菌种筛选、发酵产酶、分离纯化、克隆表达等领域。我 国起步很晚, 目前仅见中科院(北京)微生物研究所和四川大学有关于肝素酶研究 的报道。目前世界上研究最多、最深入的肝素酶仍然由肝素黄杆菌发酵生产而来。 本文通过肝素酶发酵工艺的研究，包括灭菌工艺研究、提取工艺的研究、浓缩和 干燥工艺研究及成型工艺研究，将其研制成工艺切实可行、质量稳定可控的酶制 剂。并对肝素酶制剂车间生产工艺进行研究，对工艺流程、物料衡算、热量衡算、 车间布置、管道布置、三废处理、设备造型等关键工艺进行设计，为肝素酶车间 工艺的设计提供依据。 关键词：肝素酶；车间布置；工艺流程图 Abstract In these pieces is in under the guidance of TCM theory, combined with clinical doctors the application and selected clinical experience for many years, has qingrejiedu, spleen and stomach, invigorate the circulation of bulge, clinically used for chronic gastritis, gastric ulcer, accompanied by intestinal metaplasia atypical symptoms.His own by these, spreading hedyotis herb, rhizoma coptidis, etc of ten herbs, of which one of these for his own gentleman medicine, therefore, in these extraction technology on the basis of the experimental research, the application form of his own from the traditional one, developed into taking convenient oral solid preparation, change one carry inconvenience, shortcomings and so on perishable.In this article, through these pieces of research of preparation process, including crushing and sterilization technology research, the extraction process of study, concentration and drying process and molding process research, to develop it into practical technology, stable quality control of traditional Chinese medicine compound preparations.And in these pieces tablet workshop production process, the process flow, material balance, heat balance, workshop layout, piping layout, "three wastes" treatment, equipment design on the key technology such as modelling, provide the basis for these pieces workshop