天然菌群、生物刺激和生物强化修复烃污染南极土壤的有效性研究-.doc

International Biodeterioration &Biodegradation 52(2003115– 125 /locate/ibiod E ectivenessofthe natural bacterial ora,biostimulation and bioaugmentation on the bioremediation ofa hydrocarbon contaminated Antarctic soil Lucas Ruberto a , Susana C. Vazquez a , Walter P. Mac Cormack b ; ? a Facultad de Farmacia y Bioqu? mica, Universidad de Buenos Aires, Argentina b Instituto Ant?a rtico Argentino, Cerrito 1248Buenos Aires (1010,Argentina Received 14June 2002; received in revised form 6January 2003; accepted 8January 2003 Abstract Microcosms systems (250g soil in 1l askswere performed in Jubany Station (KingGeorge Island, South Shetland Islands to analyse biodegradation ofgas-oil in Antarctic soils under natural conditions. Abiotic loss ofhydrocarbons, biodegradation activity ofindigenous micro oraand biostimulation with N and P were studied. In addition, biaugmentation with a previously isolated psychrotolerant strain (B-2-2was analysed. Hydrocarbon concentration, heterotrophic and hydrocarbon-degrading bacterial counts and predominant bacterial groups were evaluated during 51days. A signi?cantloss ofhydrocarbons was observed in abiotic control. Indigenous micro orashowed increased heterotrophic counts and hydrocarbon-degrading/heterotrophicratio. This fact was associated with a signi?cantdegrading activity (35%higher than the control. Bioaugmentation with B-2-2strain improved the bioremediation e ciency(75%ofthe hydrocarbon was removed. High levels ofN and P produced an initial inhibition ofbacterial growth. bacterial diversity was reduced in contaminated soil. Our results showed that bacterial orafrom Antarctic soils is able to degrade an important fraction of the gas-oil and that bioaugmentation represents a valuable alternative tool to improve bioremediation. ? 2003Elsevier Ltd. All rights reserved. Keywords:Bioremediation; Antarctic soils; Hydrocarbon-degrading bacteria 1. Introduction During the last two decades many investigations were performed to determine t