AOAC Liquid Chromatographic Method with Immunoaffinity Column Cleanup-国外标准规范.pdfVIP

ENTWISLE ET AL .: JOURNAL OF AOAC INTERNATIONAL VOL. 83, NO. 6, 2000 1377 FOOD CHEMICAL CONTAMINANTS Liquid Chromatographic Method with Immunoaffinity Column Cleanup for Determination of Ochratoxin A in Barley: Collaborative Study A. CATHERINE ENTWISLE, ALISON C. WILLIAMS, PETER J. MANN, and PHILIP T. SLACK Leatherhead Food RA, Randalls Rd, Leatherhead, Surrey KT22 7RY, UK JOHN GILBERT Ministry of Agriculture, Fisheries and Food, Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK Collaborators: P. Burdaspal; E. Eklund; J. Gardikis; B. Hald; M.-P. Herry; K. Jørgensen; H. Kandler; R. Maas; M.-L. Martins; S. Patel; M. Schuster; M. Solfrizzo; E. Strassmeir; R. Tiebach; T. Trogersen A collaborative study was conducted to evaluate a HORRAT values, at the low level of determination liquid chromatographic (LC) method with for ochratoxin A in barley. immunoaffinity column cleanup for determination of ochratoxin A. The method was tested at 3 con- centration levels of ochratoxin A in barley, which represent possible future European regulatory lim- ith improvements in methods of analysis for its. The test portion was extracted with ochratoxin A (particularly limits of detection), and acetonitrile–water by blending at high speed. The Wtheir wide application in dietary surveys, extract was filtered, diluted with phosphate- ochratoxin A has been found in an increasingly wide range of buffered saline (PBS), and applied to an different foodstuffs. One of the main contributing factors to ochratoxin A immunoaffinity column. The column this improvement in methodology has been the introduction of was washed with water and the ochratoxin A