大鼠烧伤早期各内脏内皮素的变化.doc

???? 大鼠烧伤早期各内脏内皮素的变化 ???? ????【摘要】　目的　探讨烧伤后内皮素变化及其在内脏损害中的作用。方法　采用大鼠30%Ⅲ度烧伤模型动态观察大鼠烧伤后血浆、心、肝、肺、肾脏内皮素的变化规律及应用非选择性内皮素受体拮抗剂PD145065对烧伤大鼠内脏损害的作用。结果　正常大鼠4种脏器每克组织中内皮素含量以肺脏最高，其次为肾、肝、心脏，血浆、肝、肾、肺脏内皮素含量伤后2小时均明显升高，6小时达峰值，24小时内仍高于伤前；心脏内皮素含量与上述变化类似，但伤后12小时达峰值，且升高幅度较其它脏器低；肝肾内皮素的升高与伤后相应肝、肾血流量减少呈明显负相关。应用PD145065不仅可使肝、肾血流量显著回升，而且明显降低心、肝、肺、肾脏器含水量，减轻内脏损害。结论　烧伤后内皮素的异常改变一定程度上参与了烧伤早期内脏损害。　　【关键词】　烧伤　内皮素 The dynamic changes and significances of endothelins in early burned rats Gao Jianchuan*，Wu Xiongfei,Yang Zongcheng,et al.*Burn Institute of People’s Liberation Army,304th Hospital,Beijing 100037　　【Abstract】　Objective　To elucidate the significance of endothelins in visceral injury at the early stage of burn.Methods　By use of a 30%(Ⅲ degree)total body surface area (TBSA) burn rat model,we measured the concentration of endothelins in plasma,heart,liver,lung,kidney,and observed the effects of PD145065,a kind of non-selective ETA-ETB receptor antagonist,on visceral impairment.Results　Not only did the endothelin concentration in plasma of burnt rat elevated significantly,showing a peak at 6 hours postburn,but also the endothelin contents in internal organs apparently increased.The changes in endothelin in liver or kidney were negatively correlated with the alteration in renal or hepatic blood flow.Moreover,administration of endothelin receptor antagonist PD145065,without affecting the arterial blood pressure,could revert the decrease of renal or hepatic blood flow to some extent,reduce the water contents of heart,liver,lung,kidney postburn.Conclusion　The abnormal changes in endothelins postburn contribute to visceral injury in the early postburn stage in rats.　　【Key words】　Burn Endothelin 　　　　内皮素是由内皮细胞合成分泌的21个氨基酸组成的小分子肽，具有广泛的生物学作用［1］，我们既往的研究发现，烧伤后血浆内皮素含量明显升高，是烧伤后毛细血管通透性改变的重要因素之一［2］，但内皮素作为一种自分泌(旁分泌)激素，它在烧伤后各脏器中的变化及其与内脏损害的关系少有报道。我们采用大鼠30%Ⅲ度烧伤模型对此加以探讨，以进一步阐明烧伤早期内脏损害的发病机理。1　材料与方法1.1　动物分组及致伤　　250±30g健康雄性Wistar大鼠50只，随机分为正常对照组(10只)及烧伤组(40只)，两组统称为伤前未动脉插管组。单笼喂养1周后烧伤组以戊巴比妥腹腔麻醉，背部脱毛后3%凝固汽油燃烧20秒，致T