灵芝多糖的药效学研究Ⅰ.抗恶性肿瘤、抗血栓和抗凝血作用.doc

???? 灵芝多糖的药效学研究?Ⅰ.抗恶性肿瘤、抗血栓和抗凝血作用 ???? ???? 摘要　本文用1.灵芝子实体经水提、乙醇沉制得灵芝多糖（GLPS）精品，用苯酚-硫酸法测其百分含量。2.应用小鼠移植性恶性肿瘤瘤株（S180、U14、EAC)，观察GLPS对荷瘤小鼠肿瘤生长的影响。3.观察GLPS对小鼠全血凝血时间及出血时间和大鼠血栓及血液凝固系统的影响。实验结果1.用本方法提取GLPS得率为0.589±0.135％。2.GLPS 40 mg／(kg．d).ig×17 d对小鼠肉瘤（S-180）和宫颈癌（U-14）的平均抑瘤率分别为43％和51％，而对艾氏腹水癌（EAC）小鼠生命没有明显延长作用（P＞0.05）。3.GLPS 20 mg／(kg.d).ig×10 d或20～40 mg.kg-1.iv均能明显延长小鼠的凝血时间和出血时间（P＜0.01)。GLPS 40 mg／(kg．d).ig×10 d能明显抑制大鼠的血栓形成（P＜0.01)，降低纤维蛋白原的含量（P＜0.01），同时能明显延长部分凝血活酶时间（KPTT）＜（P＜0.01）。说明GLPS具有抗肿瘤、抗血栓和抗凝血的作用。关键词　灵芝多糖，抗肿瘤，抗血栓，抗凝血 Studies on the Pharmacological Effects of the Polysaccharides of Ganoderma LucidumⅠ.Anticancer，Antithrombosis and anticoagulation of blood Chen Jianji　Wu Zhiqiang，et al（Fujian Medical University，Laboratory of Snake Venom Research　350004）Hu En　Lin Zhiwei et al (Fujian Colloege of Traditional Chinese Medicine 350003) Abstract　Study on the anticancer activity and the effect on blood coagulation of Polysaccharides separated Ganoderma lucidum(ling zhi)was reported in this paper.Polysaccharide of Ganoderma lucidum（GLPS）was separated by water extraction and ethanol precipitation.The yield of GLPS separated from native drug was about 0.589±0.139％ by phenol-sulfuric acid method.assayed. The cancer cell line of S-180 and U-14 were implanted into the axillary subcutaneous of mice for studying the anticancer effect.It indicated that Glps GLPS 40 mg.kg-1.ig.d×17 d decreased the weight of cancer by about 43％（S-180） and 51％（U-14）.The clotting and bleeding time of mice blood were uses for observing the effect on coagulation.The antithrombotic effect was assayed on rats by thrombosis test ex vivo.GLPS 20 mg.kg-1.d ig×10 d and 20～40 mg.kg-1.iv into mice prolonged the blood clotting and bleeding time（P＜0.01）.and GLPS 40 mg.kg-1.d.ig×10 d into rats decreased thrombosis and fibrinogen level of blood plasma（P＜0.01），the kaolin partial thrombin time was prolonged（P＜0.01）.These can conclusion that the GLPS of Ganoderma lucidum possessed the anticancer，antithrombosis and a