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METHOD 4430
SCREENING FOR POLYCHLORINATED DIBENZO-P-DIOXINS AND FURANS (PCDD/Fs) BY
ARYL HYDROCARBON-RECEPTOR PCR ASSAY
SW-846 is not intended to be an analytical training manual. Therefore, method procedures
are written based on the assumption that they will be performed by analysts who are formally
trained in at least the basic principles of chemical analysis and in the use of the subject
technology.
In addition, SW-846 methods, with the exception of required method use for the analysis
of method-defined parameters, are intended to be guidance methods which contain general
information on how to perform an analytical procedure or technique which a laboratory can use
as a basic starting point for generating its own detailed Standard Operating Procedure (SOP),
either for its own general use or for a specific project application. The performance data
included in this method are for guidance purposes only, and are not intended to be and must not
be used as absolute QC acceptance criteria for purposes of laboratory accreditation.
1.0 SCOPE AND APPLICATION
1.1 Method 4430 is a procedure for the screening of Polychlorinated dibenzo-p-dioxins
and furans (PCDD/Fs) in soil and sediment. This method uses a commercially available Aryl
hydrocarbon Receptor (AhR) based polymerase chain reaction (PCR) assay.. The AhR-PCR
assay screens samples by their toxicity equivalent quotient (TEQ) by responding to individual
PCDD/F congeners in approximate correlation to their toxicity equivalent factors (TEF). The
TEQ measured by the AhR-PCR assay is the sum of the response from the individual
congeners. See Table 2 for a detailed list of response factors for individual PCDD/F congeners.
More information about the AhR-PCR assay can be found at /dioxin/products.
1.2 The AhR-PCR assay employs AhR proteins extracted from mammalian cells to
bind PCDD/Fs. Upon binding
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