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i AS 5013.3—2009
ISO 4831:2006
Australian Standard®
Food microbiology
A
S
5
0 Method 3: Microbiology of food and animal
1
3
.
3 feeding stuffs—Horizontal method for the
—
2
0
0
detection and enumeration of coliforms—
9
Most probable number technique
PREFACE
This Standard was prepared by the Standards Australia Committee FT-024, Food Products
and Constituted Subcommittee FT-024-01, Food Microbiology, to supersede AS 5013.3—
2004, Food microbiology, Method 3: Microbiology—General guidance for the enumeration
of coliforms—Most probable number technique.
This Standard is identical with and reproduced from ISO 4831:2006, Microbiology of food
and animal feeding stuffs—Horizontal method for the detection and enumeration of
coliforms—Most probable number technique.
The objective of this Standard is to provide general guidance for the detection and the
enumeration of coliforms present in products intended for human consumption or feeding of
animals, by means of calculation of the most probable number (MPN) after incubation at
30°C or 37°C in a liquid medium, this temperature forming the subject of agreement
between parties concerned.
Clauses 4 (Principle), 9 (Procedure) and 10 (Expression of
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