高三一轮复习——动物细胞培养和核移植技术（Three rounds of review - animal cell culture and nuclear transfer techniques - 30）.doc

高三一轮复习——动物细胞培养和核移植技术-30（Three rounds of review - animal cell culture and nuclear transfer techniques - 30） Zhang pei study planning system, share daily Ii. Learning content: This week, the process of learning animal cell culture is to distinguish between the development of animal cell culture and plant tissue culture and the process and significance of animal cell nuclear transplantation 3. Study key points and difficulties Understanding the process of animal cell culture. Understand the principle of animal cell nuclear transplantation. Iv. Learning process: (1) learning content 1. Animal cell culture: cell culture is the growth of cells in vitro, and animal cells are no longer formed in the process of culture. Concept: take the tissue from the animal's body, disperse it into a single cell, and then place it in a suitable medium for these cells to grow and multiply. Ingredients: glucose, amino acid, inorganic salt, vitamin and animal serum etc. Is critical to the success of the animal cell culture medium, whether they contain animal serum, because the internal environment of animal cell lives and some components have not clear, so you need to join the animal serum to provide a similar environment of the organism, in addition to all this animal serum also contains some animal hormones and enzymes, can promote the growth of cells. The characteristics of culture medium: liquid medium and animal serum. Conditions of cultivation: sterile, non-toxic environment; Nutrient (synthetic medium); Temperature and pH (plus or minus ℃ ~); The gas environment (oxygen and carbon dioxide, animal cell culture, culture petri dishes or loose cap culture bottles in a mixture of 95% air plus 5% CO2). And so on. Basic process: To take animal embryos or young animal organs and tissues. The materials are sheared and treated with trypsin (digestion), which processes the formation of individual cells, and the individual cells are put into the medium for a certain concentration of cell suspensi