毕业论文 化橘红提取物中总黄酮测定方法比较研究.doc

目 录 TOC \h \z \t 标题1,1,标题1 + 段前: 1 行段后: 1 行 + 黑体 三号 加粗,1,标题2,2 摘要 I~ I I 1 前言 3 2 材料与仪器 5 2.1材料 5 2.2仪器 5 3 方法与结果 5 3.1紫外分光光度法 5 3.2铝盐络合法 7 3.3 SBC法 9 3.4 总黄酮含量测定 11 4 讨论 12 4.1紫外分光光度法机理 12 4.2 铝盐络合法机理 12 4.3 SBC法反应机理 12 4.4测定方法的比较 12 参考文献 14 综述 15 致谢 22 化橘红提取物中总黄酮测定方法比较研究 摘要：目的 对比3种常见总黄酮测定方法（国家食品药品监督管理局颁布的《保健食品检验与评价技术规范实施手册》收载的保健食品总黄酮测定方法(直接紫外分光光度法测定)；2010版《中国药典》收录的铝盐络合法；国际公认的较为科学合理的是美国康奈尔大学刘瑞海教授的Sodium Borohydride/Chloranil-Based Assay (简称 SBC 法)。方法 以化橘红中总黄酮的含量为指标，从精密度、回收率、重复性、稳定性方面选取最优测定方案。结果 测定结果显示铝盐络合法测得值显著大于保健食品法和SBC法测得值。结论 化橘红黄酮类成分主要为二氢黄酮成分，在碱性条件下不稳定，因此不宜应用在碱性条件下操作的芦丁比色法，应用铝盐直接与黄酮类成分显色的原理，采用柚皮苷+铝盐的方法进行总黄酮测定。测定结果显示铝盐络合法测得值显著大于紫外分光光度法和SBC法测得值。 关键词：化橘红；黄酮；测定方法 Study on Total?flavonoids?in?Exocarpium citri Grandis?extract?comparative method Abstract：Objective Comparison of 3 methods for the determination of total flavonoids?(common?method usedmanual health food?of total flavonoids of implementing technical standards for testing and assessment of?health food was promugated by the State Food and Drug Administration?(?bydirect ultraviolet spectrophotometry);?2010 Edition?Pharmacopoeia included Chinesealuminum salt?complexing;?internationally recognized?more scientific and reasonable?is a professor at the?Cornell University?Liu Ruihai?Sodium?Borohydrid/Chloranil-Based?Assay America?(SBC)).?Select the best?from the?precision, recovery rate,?stability and other aspects of?measurement. Methods The content?of?total flavonoids?in?orange?as the?indicator,?the?precision,?recovery, repeatability,?stability?determination of?optimal selection of?schemes.Results The results showed?the aluminum salt?complexing?measured value?was significantly higher than that of?health food?and SBC?measured value.Conclusion Flavonois? with?polyamide column chromatography?are selectively enriched?containing phenolic hydroxyl?radicals?by UV spectrophotometry,?without?color?determination, the