U6_5S_miRNA引物探针设计.docx

内参 1. RNU6 Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA NR_004394.1 ORIGIN 1 gtgctcgcttcggcagcacatatactaaaattggaacgatacagagaagattagcatggc 61 ccctgcgcaaggatgacacgcaaattcgtgaagcgttccatatttt RNU6-F CTCGCTTCGGCAGCACA RNU6-R AACGCTTCACGAATTTGCGT PrijTib 山 弓■que~nt? Length Tm GCh totnpIkimctiiE li^ S?ir 3' cCMpllt rnvriitJirity F a ward pr| mtr C TCGCITCGGCAGOMOA 17 即网 6d.?1 3 DO R*vtr?* pirmtl AWGCTTC^GAAI 1 T&CGT 2Q "前 4d.DQ 9.DD 加g Product's on tai gat NIR 1 Hand sapiens RHA.. LEi small nuclEar 7 [RMUE-r). small nuclear RNA product 】K^；m ■ H FWgd priner 1 CTCiXTT^CCAGClCA IT Teripl-ite 」 刘 Rr.wse jrlner 1 &aOGCTTCjiC&iaTTTGCGT 2州 2. 5S Homo sapiens RNA, 5S ribosomal 2 (RNA5S2), ribosomal RNA NR_023364.1 ORIGIN 1 gtctacggccataccaccctgaacgcgcccgatctcgtctgatctcggaagctaagcagg 61 gtcgggcctggttagtacttggatgggagaccgcctgggaataccgggtgctgtaggctt 121 t 5S-F TCTCGTCTGATCTCGGAAGCTA 5S-R GCGGTCTCCCATCCAAGTA Prjmftr pair 1 Length Tm GC% Self co-rnpmiriwntiinty 3* c ompl Fvrwihni pnnw ICTCGTC TGATC FCGOhAjGCTA H 60.帼 5D.OO 4JD 2.QG Reverie pnmtr GOiSGTCTCCiCArccAAOTA 迥 Sfl 四 S7 eg 3.DD 2QC target tsimpEMes ? H .—北＞ i nwhi 如血nra 曲A,姑 HIjl 顽rB I (RrtASE IJi, hltoscrhal Rr^ l甄eUl = 6E F.jRTircL prliex 1 TCTCCmmTCT询UGCTA 22 T诲33 Rrvet-se prlier 1 G翊TCIOSOTC3GTA 19 Iflhplnte 54- .........i......... 76 MiRNA 方案1 Reverse primer TGGTGTCGTGGAGTCG RTadaptor： CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAG F o rward adapt or: RGACTGCAiXTg G LenQth of RTprimer' 8 Optimuiri length of foiward primeit 15 Minimu m length of forward primer: 13 Maximium lengtM of forward primer. 17 RT primer adaptor CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGXXXXXX Forward primer adaptor ACACTCCAGCTGGGXXXXXXXXXXXXXX Universal Reverse primer TGGTGTCGTGGAGTCG 方案2 Reverse primer- ATC C AGT0 C AC 6 GTC CO AGO RT adaptor GTCGTATCCMTGCAGGGTCCGgWWGCMTGGATMGM Forward adaptor： GCGGCGG Length jfRT primer 6 Optimum length o(Tforward primer 15 Minimunn length offsNvard primer 13 Ma}<irnum length of forward primer 17 RT prim