幽门螺杆 菌全菌蛋白抗原壳聚糖微球体外释放特性.doc

幽门螺杆菌全菌蛋白抗原壳聚糖微球体外释放特性*★ 刘 微1,2,谢 　勇2,刘志军１ Prepaｒaｔion aｎd in viｔrｏ relｅase cｈaｒａｃterｉｓtiｃs　of ｃhitosａn　ｍicrosphereｓ　lｏadiｎg Helicｏbacter　ｐｙｌori whoｌｅ ceｌl ｐroteiｎ anｔｉgen Liu Wei1,２, Xie　Ｙong2,　Lｉu Zhi-jun１ 1Digestive System Department, People's Hospital of Xinyu, Xinyu 338025, Jiangxi Province, China; 1Digestive System Department, People's Hospital of Xinyu, Xinyu 338025, Jiangxi Province, China; 2Institute of Digestive Disease, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China Liu Wei★, Master, Physician, Digestive System Department, People's Hospital of Xinyu, Xinyu 338025, Jiangxi Province, China; Institute of Digestive Disease, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China baibianfuliuwei 163、 Correspondence to: Xie Yong, Professor, Chief physician, Institute of Digestive Disease, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China xieyong_med 163、 Supported by: the National Natural Science Foundation of China, No、 Received: 2010-10-05 Accepted: 201 新余市人民医院消化内科,江西省新余市 338025;2南昌大学第一附属医院消化疾病研究所,江西省消化疾病研究重点实验室,江西省南昌市 330006 刘微,男,1983年生,江西省新余市人,汉族,2010年南昌大学医学院毕业,硕士,医师,主要从事幽门螺杆菌感染免疫致病与免疫防治。 通讯作者:谢勇,教授,主任医师,南昌大学第一附属医院消化系病研究所,江西省消化疾病研究重点实验室,江西省南昌市 330006 xieyong_med 163、 中图分类号:R318 文献标识码:B 文章编号:1673-8225 (2011)29-0000-04 收稿日期:2010-10-05 修回日期:2011-03-12 (20101005006/M ?W) BAＣKGROＵND:　Ｃhitoｓan ｍiｃrｏspherｅs lｏaｄing Helｉcobacter pｙloｒｉ whｏlｅ　cell protein aｎtigen　pｒepaｒed　by ｄｉffereｎt　ｍethods hａvｅ diｆfｅrent　ｔhe paｃkage　ｒaｔeｓ aｎd controlled—ｒelease　eｆfeｃｔs。 OBJＥＣＴＩVＥ: To　ｐrｅpａre chitｏｓan micrｏspheres　loａdｉｎg Heｌicobａctｅr ｐyloｒi ｗhoｌe cｅlｌ proｔein antiｇen anｄ　obsｅrve the ｒeｌeasｅ　chａｒaｃterｉstics in vｉtro. MEＴHODS: Berｔhold prｅcipitatｉｏn metｈｏd　was uｓeｄ　ｔo pｒepare chｉｔｏｓａｎ　microsphereｓ and　thｅ ｐｒeparation was optimｉｚed; scannｉng elｅctron mｉcｒoscope　aｎd pａrtiｃｌｅ size　ａｎalyzｅr wｅrｅ usｅｄ　ｔｏ obｓerｖe the ｓhaｐｅ and size me