谢建ZnPP对种植性乳腺癌血管生成影响的实验研究应用.doc

·大会交流· ZnPP对种植性乳腺癌血管生成影响试验研究 谢建 杨渊 刘胜春 400016 重庆，重庆医科大学隶属第一医院普外科 　 【摘要】目标 探讨锌原卟啉（ZnPP）对种植性乳腺癌VEGF和血管生成影响。方法 应用种植性乳腺癌模型，经过ZnPP和钴原卟啉（CoPP）处理，检测乳腺癌组织中血红素氧合酶1(HO-1)酶活性，免疫组化、Western blot蛋白印迹杂交检测癌组织中HO-1、VEGF蛋白表示，RT-PCR检测VEGFmRNA，CD34染色肿瘤微血管内皮细胞并对其进行定量分析。结果 ZnPP组肿瘤直径（7±1.06mm，n=7，）显著小于其它各组(t=3.5334 ,P<0.001 )；CoPP组（13±2.82 mm，n=13，）大于空白对照组（10±1.63mm，n=8） (t=3.6564, P<0.002)。CoPP组VEGFmRNA (0.3046±0.0102)显著高于空白对照(0.1524±0.0046)及ZnPP组(0.1216±0.0043)（t=8.8491,P<0.001）。 ZnPP处理组HO-1及VEGF蛋白印迹杂交结果低于CoPP组和空白对照组（t=4.698,P<0.005）（t=5.5428,P<0.005）。ZnPP处理组MEA为224.81±79.4266显著低于CoPP组448.79±138.46 (t=5.3426, P<0.05)。结论 ZnPP可能经过抑制HO-1蛋白表示来降低VEGF表示，降低肿瘤血管生成，并可能由此降低了肿瘤细胞增殖生长。 【关键词】血红素氧合酶-1；乳腺肿瘤；血管内皮细胞生长因子；血管生成 Experiment study of Effect of Zinc Protoporphyrin on angiogenesis in planted breast carcinoma XIE Jian,YANG Yuan,LIU Sheng-chun. Department of General Surgery, The First Affiliated Hospital, Chongqing University of Medical Science, Chongqing 400016,China 【Abstract】Objective To investigate the effect of Zinc Protoporphyrin(ZnPP) on VEGF and angiogenesis in planted breast carcinoma. Methods The planted breast carcinoma was treated with ZnPP and Cobalt Protoporphyrin(CoPP), the Haem oxygenase-1(HO-1) protein and enzyme activity in carcinoma tissue were assessed by western blot and so on. At the same time VEGF were examined by RT-PCR, western blot and microvessel endothelial cell were stained by immunohistochemstery with CD34. Microvessel Endothelial Area(MEA) were estated. Results The diameter of tumor in ZnPP group was significantly smaller than that in other groups (t=3.5334 ,P<0.001 ) and that in CoPP group was biggest(t=3.6564, P<0.002)。The VEGF mRNA in tumor of ZnPP group(0.1216±0.0043) were lower than those in other groups, and which in CoPP group were highest(0.3046±0.0102)（t=8.8491,P<0.001）. The MEA in ZnPP group (224.81±79.4266) were lower than those in CoPP group(448.79±138.46）(t=5.3426, p<0.05). Conclusions ZnPP is likely to inhibit HO-1 pr