NGF,NaHS对哮喘大鼠气道炎症的调节作用.docVIP

NGF、NaHS对哮喘大鼠气道炎症的调节作用 陈巧彬 陈琅 目的 观察卵白蛋白（OVA）诱导建立的急性支气管哮喘大鼠，研究神经生长因子（NGF）及外源性硫化氢钠（NaHS即H2S供体）处理后哮喘大鼠肺中粘蛋白基因的表达情况，探讨它们在哮喘的炎症中起到的作用。方法 36只健康30日龄SD大鼠按照随机数字表法分为正常对照组、哮喘组、NGF干预组及NaHS干预组，每组分别为8只、9只、10只、9只。激发后24小时解剖大鼠，用免疫组化的方法测定大鼠肺中粘蛋白基因Muc5ac的表达情况。结果 正常对照组，哮喘组及NGF干预组及NaHS干预组肺Muc5ac蛋白含量（用阳性面积表示）分别为501.75±270.58；1330.22±662.87；2033.90±892.28；715.88±282.49 平方微米。4组间差异有统计学意义(F值为11.82，p0.01) 结论 哮喘组大鼠粘蛋白基因Muc5ac表达增加，NGF进一步加重气道炎症，而外源性硫化氢可以减轻哮喘气道炎症。 【关键词】鼠神经生长因子；硫化氢；哮喘；大鼠；粘蛋白基因；卵白蛋白 [abstract] Objective: To explore the effect of gaseous signal molecule hydrogen sulfide (H2S) and nerve growth factor (NGF)on the lung Muc5ac espresses in OVA-induced asthma rats. Method: 36 healthy SD rats were randomly divided into four groups： control group （n=8）, asthma group (OVA induced, n=9), a NGF group( OVA induced rats treated with NGF n=10) and a NaHS group(OVA induced rats treated with NaHS, n=9). 24 hours of treatment, rats were anatomized to observe The express of lung Muc5ac were determined by immunohistochemical staining. Result: The expression of lung Muc5ac, expressed with positive areas, were 501.75±270.58um2,1330.22±662.87um2, 2033.90±892.28um2,715.88±282.49um2 separately in control group, asthma group , asthma group treated with NGF and asthma group treated with NaHS. There are dramatically statistics significance among four groups(F=11.82, P0.01). Conclusion :The express of lung Muc5ac increase in OVA-induced asthma rat. NGF may play an important role in worsen airway inflammation . But exogenous hydrogen sulfide may play an important role in alleviate airway inflammation and protect from asthma acute break out. KEY WORDS hydrogen sulfide; nerve growth factor; Asthma; Rat; Muc5ac; OVA 哮喘是一种多基因遗传疾病，哮喘的发病机制复杂，调控因素众多，但最终引起临床疾病过程的关键是气道的炎症。NGF、NaHS对哮喘大鼠气道炎症起到一定的调节作用。 1．材料与方法 1.1动物分组及处理 健康SD雄性大鼠（上海斯莱克实验动物有限责任公司）36只，近交系清洁级，鼠龄30日，平均体重280±20g，按照随机数字法分组，分为对照组（n=8）、哮喘组（n=9）、NGF干预组（n=10）及NaHS组（n=9）。 1.1.1哮喘组：分别于第1天，第8天用新鲜配制的卵白蛋白（OVA）10mg＋氢氧化铝200mg＋生理盐水1ml进行腹腔注射致敏。 实验的第15至第22天，将实验动物装在30×20×