紫洋葱中抗氧化花色苷的快速鉴别技术研究.docVIP

紫洋葱中抗氧化花色昔的快速鉴别技术研究 傅茂润舄赵双t刘玉芹2,何志平歹 1.山东轻工业学院食品与生物工程学院（济南250353）； 2.山东阿如拉医药发展有限公司（济南250100）； 3.浙江农林大学食品学院（临安引1300） 摘要建立一种能够快速，准确的分析鉴别并比较具有抗氧化活性的花色昔的方法。［以紫洋葱为研究 对象，采用DPPH口由慕柱前衍生化片HPLC-MS联用技术分析具有抗氧化活性的花色苛物质特征峰，然 后采用液质联用技术对英进行结构鉴定。从紫洋葱屮鉴定出6种花色甘，并对英构效关系进行了分析，各 个花色琶组分均具有较强的抗氧化能力，但存在一定的区别，英屮矢车菊-3,5■二葡萄糖百的抗氧化能力 最强,矢车菊?3?葡萄糖甘，矢车菊?3?丙二酸酰葡萄糖琶和勺药?3?丙二酸酰葡萄糖百的抗氧化能力次之， 而矢车菊-3,5-M二酸酰二葡萄糖琶和芍药?3,5■内二酸酰二葡萄糖苛的较弱。该方法的建立，有助于实现花 色昔类物质抗氧化活性的快速比较和鉴定，为揭示其构效关系提供理论和技术支持。 关键词抗氧化活性；花色莒；柱前衍生化反应；液质联用；紫洋葱；构效关系 Rapid Identification and Comparison of Antioxidant Activity Anthocyanins in Red Onion Fu Mao-run1, Zhao Shuang1, Liu Yu-qin2, He Zhi-ping3* 1? College of Food Science and Bioengineering, Shandong Polytechnic University (Jinan 250353); 2. ShandongAiura Pharmaceutical Reseaich Development Li mi led Co. (Jinan 250100); 3. Department of Food Science, Zhejiang Forestry College (Linan 311300) Abstract To established a new method, which could analyze, identify and compare the antioxidant activity quickly and accurately. Red onion was employed as ex peri mental material, combination of pre-colum n derivatization reaction with DPPH (1, 1 -diphenyl-2-picrylhydrazyl) free radical and HPLC-MS was used to analyze the characteristic peaks of anthocyanins in red onion with antioxidaactivity, then, structure identification was done by HPLC-MS? 6 anthocyanins compounds was identified in red onion and the relationship between structure and antioxidant activity was expanded? Results showed these compounds had high antioxidant activity, however, difference in activity was occurred that cyan-3,5-diglucoside had the highest antioxidant activity, followed by cyanidin-3?O?glucoside、 cyanidin-3-malony 1-glucoside and peonidin 3-nialony 1-glycoside, and cyanidin -3,5-malon yl-diglucoside and peonidin -3,5? malonyI-diglucoside was the lowest? Establishment of the method would help to identify anthocyanins compounds quickly, which provided theory and technical support for anthocyanins compounds to investigate their