环庚三烯酮合成（7元环） Shunting Phenylacetic Acid Catabolism for Tropone Biosynthesis.pdfVIP

Research Article Cite This: ACS Synth. Biol. 2019, 8, 876−883 pubs.acs.org/synthbio Shunting Phenylacetic Acid Catabolism for Tropone Biosynthesis Yan Li,† Mengyuan Wang,† Qianjing Zhao,† Xiaolin Shen,† Jia Wang, † Yajun Yan,‡ Xinxiao Sun,*,† and Qipeng Yuan*,† †Beijing Advanced Innovation Center for Soft Matter Science and Engineering, State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China ‡School of Chemical, Materials and Biomedical Engineering, College of Engineering, The University of Georgia, Athens, Georgia 30602, United States S *Supporting Information . s e l c i t ABSTRACT: Tropone is a seven-membered ring non- r a d benzenoid aromatic compound. It is the core structure of e h s tropolonoids, which have various biological activities. In this i l . b ) u study, a hybrid tropone biosynthetic pathway was designed by C p T e connecting phenylacetic acid (PAA) degradation with its U r ( a 4 h biosynthesis and reconstituted in Escherichia coli. To simplify s 1 : y 5 l pathway construction and optimization, the use of E. coli 2 e t : a 6 endogenous genes was maximized and only three exogenous 0 m i t t a