现化专题孔老师课件单细胞电化学.pptx

;单细胞水平分析特点; 单细胞/单分子水平检测的电化学分析技术;细胞化学组成的in-vitro电化学研究 单活细胞化学组成的in-vivo电化学研究 单活细胞生理行为的电化学研究 细胞药物代谢动力学过程在线分析;;;Kinetic amperometric I~ t response to NO in a single cell with a TTP-modified micro-fiber electrode;;皂角苷刺激嘌呤释放; 超微电极技术----单活细胞化学组成;Figure 3. Dynamics of doxorubicin adsorption onto a stabilized CFDE surface in oxygen-free efflux medium containing 1 uM doxorubicin. OSWV from -300 to -800 mV at 800 mV/s was applied to detect the cathodic peak after a freshly stabilized CFDE was immersed into the solution. The inset shows individual OSWV sweeps obtained at every 4 s. The diamonds represent the heights of the differential current peak determined from the OSWV curves. The nominal time of electrode immersion is at t) 0. Solid line: exponential saturation curve least-squares fitted to the data.;Figure 1. Schematic diagram of the experimental arrangement for electrochemical detection of drug efflux from a single cell using a carbon fiber microdisk electrode (CFDE). (A) Schematics of the setup used for efflux detection. A single cancer cell, which is at least 150 ím away from other cells, is chosen for any experiment. The CFDE is moved to the selected cell and held at ￠ 1 um away by a three-axis hydraulic micromanipulator. A Ag/AgCl wire is used as the reference. (B) Schematic close view of the studied cell and the tip of the CFDE, displayed to scale with approximate dimensions. (C) Transmittance images of both CHR C5 and AUXB1 cells during efflux detection with a CFDE. (The 20-um bar relates to both panels B and C except that the height of the cell in (B) is exaggerated.);Figure 1. (A) Scanning electron micrograph of the oxygen microsensor tip, prepared as described in the Experimental Section. (B) Illustration of oxygen microsensor construction. Dimensions are given in micrometers.; A microcolumn liquid chromatography method for determining norepinephrine (NE), epinephrine (E), and phenylethanolamine N-methyltransferase (PNMT) enzyme activity in si