甲状腺功能亢进肝阳上亢证大鼠下丘脑组织蛋白质差异表达的观察.doc

甲状腺功能亢进肝阳上亢证大鼠下丘脑组织蛋白质差异表达的观察 作者：周凌燕,陈泽奇,李炜,李智,颜永平 【摘要】 目的 利用蛋白质组学方法,观察甲状腺功能亢进(简称“甲亢”)肝阳上亢证大鼠与正常大鼠下丘脑组织的双向凝胶蛋白图谱,寻找差异表达的蛋白质。方法 左甲状腺素钠(L-T4)和附子汤灌胃造成甲亢肝阳上亢证模型,应用蛋白质组学技术比较模型组与正常组中下丘脑的差异蛋白质,结合生物信息学对其进行分析鉴定。结果 两组蛋白斑点总体分布相似,主要分布在等电点pI 3～8,分子量Mr 14.4～75 kD；分析发现,模型组与正常组相比有18个蛋白质上调和24个蛋白质下调,进一步质谱鉴定,其中7个蛋白质分别为NSFL1 辅助因子、硫氧还蛋白、泛素羧基末端水解酶同工酶L1、血小板活化因子乙酰基水解酶IB-γ亚单位、谷氨酸脱氢酶前体、二氢嘧啶酶相关蛋白2和微管蛋白。结论 模型组与正常组相比,有18个蛋白质点上调,有24个蛋白质点下调,经质谱鉴定了其中部分蛋白质。蛋白质的变化可能与甲亢肝阳上亢证的形成密切相关。 【关键词】 甲状腺功能亢进；肝阳上亢证；下丘脑；蛋白质组学；动物模型 Observation of Differential Protein of Hypothalamus in the Hyperthyroidism Rats with Hyperactivity of Liver-Yang ZHOU Ling-yan, CHEN Ze-qi, LI Wei, et al (Chinese Traditional and Westenr Modern Medicine Research Institute of Xiangya Hospital, Central South University, Changsha 410008, China Abstract：Objective Utilization of proteome method, by observing differences on protein expression of hypothalamus in the hyperthyroidism rats with hyperactivity of liver-yang and normal rats, to search the differential protein. Methods Models of animal were prepared by ip L-T4 and Fuzi Decoction. Both the quantitative and qualitative changes of the protein expression were compared between model group and treated group by protein technique. Results Proteins were mainly displayed at range of pI 3～8, Mr (14.4～75)kD. 18 protein increased and 24 protein decreased in model group compared with treated group, included Thioredoxin, Ubiquitin carboxyl-terminal hydrolase isozyme L1, Platelet-activating factor acetylhydrolase IB gamma subunit, glutamate dehydrogenase, dihydropyrimidinase-related protein 2, NSFL1 cofactor p47, Tubulin beta 5. Conclusion There were 18 spots high expressed and 24 spots low expressed in the hyperthyroidism with hyperactivity of liver-yang, that maybe related with the 甲状腺功能亢进(简称“甲亢”)是由于多种病因引起甲状腺激素分泌过多,而导致基础代谢增加、自主神经功能失常、甲状腺肿大等为特征的常见内分泌疾病。在中医学中,甲亢属于瘿病范畴,早在公元前3世纪,就已有关于瘿病的记载。目前,临床上各医家对甲亢的辨证分型各不相同,但大多数认为肝阳上亢证为甲亢常见证型[1-2]。由于甲状腺功能主要受下丘脑、垂体的调节,因此,笔者拟应用蛋白质组学技术,通过观察甲亢肝阳上亢证大鼠与正常大鼠下丘脑组织蛋白质双向