微波对骨细胞活力和生物力学的影响.doc

微波对骨细胞活力和生物力学的影响.doc

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微波对骨细胞活力和生物力学的影响 目录 TOC \o "1-9" \h \z \u 目录 1 正文 1 文1:微波对骨细胞活力和生物力学的影响 1 1 材料和方法 2 2 结果 4 3 讨论 5 文2:床边教学对骨科医学教学的影响 6 1床边教学的目的及影响因素 6 2床边教学的方法与要求 7 3加强沟通能力的培养是提高床边教学质量的关键 10 4结束语 13 参考文摘引言: 13 原创性声明(模板) 14 文章致谢(模板) 15 正文 微波对骨细胞活力和生物力学的影响 文1:微波对骨细胞活力和生物力学的影响 【Abstract】Objective To investigate the effects of microwave induced hyperthermia on cell viability and biomechanics of bone cells in rat's femur. The biomechanics varieties of rat's femur after complete eradication of all viable cells in rat bone were studied. Methods 32 rats were euthanized and femu were removed by disarticulated. The left femu were heated for 3 minutes, 5 minutes, 10 minutes and 15 minutes with the microwave and the histochemical assay for special lactate dehydrogenase activity was evaluated. The rat bone's biomechanics and the content of calcium tests were carried out. The right femu were taken as control. Results There was no significant difference in rat bone biomechanics and the content of calcium between both groups (p>) and complete cell death occurred after 10 minutes of heating at 62℃. Conclusion It was found that complete eradication of all viable cells in rats bone could be achieved with minimal reduction mechanical function in shorter time by microwave induced hyperthermia. The method to determine osteocyte's viability by histochemical assay for lactate dehydrogenase activity proves to be seitive and specific. Microwave may be applied in limb salvage surgery on patients of bone tumor. 【Key words】Microwave Cell viability Biomechanics Bone Lactate dehydrogenase 本研究旨在探讨微波加热对骨、软骨细胞活力及骨组织生物力学的影响,最终为临床提供理想的骨肿瘤区段处理方法。 1 材料和方法 取材及实验分组 32只8周龄健康雄性Wister大鼠(山东大学动物中心提供),体重180~300g,处死后行关节离断,完整取出股骨,仔细分离去除软组织(不损伤骨表面),自中点用电锯横断一分为二(为使温度计探针能进入髓腔内),用生理盐水冲洗血液及髓内组织后,放入生理盐水中备用。左侧股骨为实验组,右侧股骨为对照组(除不加热外,其余过程同实验组) 微波加热 将左侧的64块半股骨随机分为Ⅰ~Ⅳ组,每组16块,各组的加热时间分别为3、5、10,15min。加热方法:(1)预加热试验:将Ⅰ~Ⅳ组骨块,每组随机取出2块,共8块放入100×15mm的培养皿中,加入40ml生理盐水将其浸没,用2450MHz微波机(WE2102-A,北京产)加热,输出功率调为160W,将

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