survivin反义寡核苷酸激活caspase3诱导SGC7901胃癌细胞凋亡的实验研究临床医学.doc

survivin反义寡核苷酸激活caspase3诱导SGC7901胃癌细胞凋亡的实验研究临床医学 目录 TOC \o "1-9" \h \z \u 目录 1 正文 1 文1：survivin反义寡核苷酸激活caspase3诱导SGC7901胃癌细胞凋亡的实验研究临床医学 1 1 材料和方法 3 2 结 果 5 3 讨 论 6 文2：survivin反义寡核苷酸诱导胃癌原代细胞凋亡的实验研究临床医学 7 1 材料与方法 9 2 结果 11 3 讨论 12 参考文摘引言： 13 原创性声明（模板） 15 文章致谢（模板） 15 正文 survivin反义寡核苷酸激活caspase3诱导SGC7901胃癌细胞凋亡的实验研究临床医学 文1：survivin反义寡核苷酸激活caspase3诱导SGC7901胃癌细胞凋亡的实验研究临床医学 Abstract: Objective To investigate the effect of trafected survivin antisee oligonucleotide on inducing apoptosis in gastric cancer cells and the possible causative mechanism. Methods The experiment included four groups: control, liposome (Lip), see oligonucleotide (Sodn) and antisee oligonucleotide (Asodn). Artificially synthesized survivin antisee oligonucleotide was trafected for 48 hou into gastric cancer cells using liposome. After cultivation, the cell proliferation inhibition rate was evaluated by MTT assay, the expression of caspase-3 protein was determined with Western blot, and the morphological changes of apoptosis were observed by Hoechst staining, and the apoptosis rate was tested with Annexin v-FITC assay of flow cytometric analysis. Results Fluorescence microscopic observatio revealed that Hoechst staining in the control, Lip and Sodn groups had nucleus presented as normal blue, while the Asodn group had such changes as reinforced nucleus staining, karyoplasm concentration, karyorrhexis and apoptotic modificatio. Asodn group had a significant increase in the cell proliferation inhibition rate, cell apoptosis rate and expression of caspase-3 protein, compared with the control, Lip and Sodn groups. The differences had statistical significance (P<). Conclusion Survivin antisee oligonucleotide can induce apoptosis of gastric cancer cells, Its possible mechanism might be that the apoptosis signal traduction is initiated by the activation of caspase-3 expression. Key words: survivin; antisee oligonucleotide; caspase-3; gastric cancer cell; apoptosis 英文 已换 生存