脉冲电磁场结合碱性成纤维细胞生长因子促进软骨修复的实验研究（临床医学资料）.doc

脉冲电磁场结合碱性成纤维细胞生长因子促进软骨修复的实验研究（临床医学资料） 文档信息 属性： F-01NHT1，doc格式，正文28091字。质优实惠，欢迎下载！ 适用： 作为医学资料、临床医学资料写作的参考文献，解决如何写好实用应用文、正确编写文案格式、内容摘取等相关工作。 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：脉冲电磁场结合碱性成纤维细胞生长因子促进软骨修复的实验研究 2 1 材料与方法 3 2 结果 4 3 讨论 6 文2：碱性成纤维细胞生长因子促进兔缺血后肢血管新生的实验研究 7 1 材料和方法 8 2 结 果 10 3 讨 论 11 参考文摘引言： 12 原创性声明（模板） 13 文章致谢（模板） 14 正文 脉冲电磁场结合碱性成纤维细胞生长因子促进软骨修复的实验研究（临床医学资料） 文1：脉冲电磁场结合碱性成纤维细胞生长因子促进软骨修复的实验研究 Abstract：To study mechanism of the cartilage repair with pulsed electromagnetical fields combined with b-fibral growth New Zealand white rabbits were devided into 4 groups as bFGF group,　magnetic group, pulsed electromagnetical fields combined with b-fibral growth factor group and nontreatment group randomly. The animals were sacrificed at 4、8、12 and 16 weeks after treatment. The specime were examined with the gross, histological and TEM evaluation . Results showed that four weeks after treatment,cartilage-like tissue grew at the bottom. The cartilage of germinative layer and basal layer grew better than that in bFGF group, magnetic group. Chondrocytes grew better and secreted collagen Ⅱ, while the cartilage –like tissue proliferated less. At 8 and 12 weeks, fibrous cartilage in combined group was thicker than that in other groups, and the cells were larger and muturer and more collagen Ⅱ.At 16 weeks, the cartilage was similar to normal one. The chondrocytes were muturer. Wakitani score of cartilage repair was significantly different in all groups, it was better in the combined group at 16 repairing defects with pulsed electromagnetical fields combined with b-fibral growth factor is a promising method. It can be used in clinic in the future. Key words：Pulsed electromagnetical fields；B-fibral growth factor；Cartilage defect repair 由创伤和退行性病变引起的骨关节炎是临床常见的疾病，其治疗是临床的难题之一。常见的治疗方法有钻孔术、微骨折、骨膜和软骨膜移植、细胞移植、骨软骨移植等。其结果是纤维软骨修复或透明样软骨修复，很快退变，治疗效果不理想［1］。利用细胞生长因子、基因转染等［2］促进软骨修复是近年来软骨组织工程研究的热点。我们用低强度脉冲电磁场结合碱性成纤维细胞生长因子治疗软骨缺损，取得较好疗效。现报告如下。 1 材料与方法 40只新西兰