MBL基因突变检测新方法及RRTI患者的突变研究（临床医学资料）.doc

MBL基因突变检测新方法及RRTI患者的突变研究（临床医学资料） 文档信息 属性： F-01NHT2，doc格式，正文30039字。质优实惠，欢迎下载！ 适用： 作为医学资料、临床医学资料写作的参考文献，解决如何写好实用应用文、正确编写文案格式、内容摘取等相关工作。 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：MBL基因突变检测新方法及RRTI患者的突变研究 1 1 材料与方法 3 2 结果 5 5:空白对照(dH2O取代模版DNA) 5 3 讨论 6 文2：散发性帕金森病Parkin基因突变检测 8 1资料和方法 9 参考文摘引言： 12 原创性声明（模板） 13 文章致谢（模板） 13 正文 MBL基因突变检测新方法及RRTI患者的突变研究（临床医学资料） 文1：MBL基因突变检测新方法及RRTI患者的突变研究 [ABSTRACT] Objective: To develop a rapid and seitive method for detecting MBL genetic mutants and evaluate the association with recurrent respiratory tract infectio(RRTI)in children. Methods: The MBL exon1 region was amplified by PCR, the nonincorporated prime and excesses dNTPs were removed by enzymatic digestion. The dyeterminator (R110acyC or TAMRAacyT) was incorporated on the 3′end of the primer via template directed dyeterminator incorporation reaction (TDI) and the mutatio were determined by fluorescence polarization (FP) assay. The MBL mutants were analyzed among 50 healthy and 46 RRTI children using this new method. Results: The accuracy and seitivity were evaluated and verified by standard sequencing method. The 54 GA mutant was found among both healthy and RRTI children. The genotypes among healthy group were: 39 homozygote wildtype G/G (%), 8 heterozygote G/A (%) and 3 homozygote mutant A/A (%). The genotypes among the RRTI children were: 18 homozygote wildtype G/G (%), 22 heterozygote G/A (%) and 6 homozygote mutant T/T (%). The frequency of MBL mutation was significant higher in RRTI children than in healthy controls. The 52CT and 57GA mutants were not found in both groups. Conclusio: The MBL mutation associates with the risk of RRTI among children. This new MBL genotype can be used to help the diagnosis and screening the high risk children for RRTI. [KEY WORDS] Mannanbinding lectin;Genetic mutant; Recurrent respiratory tract infectio; Fluorescence polarization 甘露聚糖结合凝集素(mannanbinding lectin, MBL)是血浆中的胶原凝集素，其多糖识别结构域与细菌、真菌及寄生虫等表面的甘露糖或N乙酰氨