聚合酶链式反应扩增.pptxVIP

会计学;二、实验原理(Experimental Principle);Polymerase chain reaction (PCR) is used to amplify a sequence of DNA, using a pair of oligonucleotide primers each complementary to one end of the DNA target sequence. These are extended towards each other by a thermostable DNA polymerase in a reaction cycle of three steps: denaturation of template, annealing of primers and extension of the primers.; As figure explains, this technique uses enzyme DNA polymerase to make a copy of a defined region of DNA . ; First a high temperature (about 95℃) to separate the DNA strands;（首先，高温(约95 ℃)使 DNA双链解离）;A typical