重庆巫山独活最佳采收期研究.docx

PAGE PAGE 1 重庆巫山独活最佳采收期研究 重庆巫山独活最正确采收期讨论5304〔2021〕06-0070-05 Abstract： Objective To compare yield characteristics， contents of osthole and columbianadin of Angelicae Pubescentis Radix of different collecting time in Wushan of Chongqing; To provide references for reasonable collecting time. Methods Adopting small plot experiment in the field， taking the main root length， root diameter， fresh root weight， dry root weight and drying rate as investigation basis， collecting time from September 5th 2021 to March 3rd 2021 was divided into ten collecting time， collecting every 20 d. Taking osthole and columbianadin as the index components， the HPLC was used to determine them， and the principal component analysis method was used for comprehensive evaluation. Results Root length， root diameter， fresh root weight， dry root weight and drying rate increased first then decreased along with the collecting time. Osthole increased first， then decreased gradually after reaching a peak value in the middle of December. The change of columbianadin was a quadratic curve with the collecting time， and reached the peak at the end of November and mid-February， but there was not significant difference. Conclusion The fresh root weight and contents of osthole and columbianadin in Angelicae Pubescentis Radix has a certain relationship with the collecting time， combined with the fresh root weight and index components， the optimum collecting time of Angelicae Pubescentis Radix in Wushan of Chongqing is mid-December， which can provide theoretical basis for the choice of collecting time of Angelicae Pubescentis Radix. Keywords： Angelicae Pubescentis Radix; collecting time; yield characteristics; osthole; columbianadin; HPLC; content determination 獨活是伞形科植物重齿毛当归Angelica pubeseeas Maxim. f. biserrata Shan et Yuan的枯燥根，始载于《神农本草经》，列为上品，具祛风除湿、通痹止痛之成效[1]。迄今为止，国内外学者对独活资源[2]、本草考证[3-5]、化学成分[6-10]、药理作用[11]、临床应用[12]和栽培技术[13-15]等方面进行了大量讨论，其讨论结果对独活资源的有效利用具有重要的理论价值。采收期对中药材的产量、有效成重量、临床疗效等方面均有直接影响[16]，202