Agilent Purification of antibodies from cell culture supernatant using the Agilent AssayMAP Bravo platform说明书用户手册.PDF

Purification of antibodies from cell culture supernatant using the Agilent AssayMAP Bravo platform Application Note BioPharma Authors Abstract David Knorr, Marc Beban The Agilent AssayMAP Bravo platform has been developed to automate a variety Agilent Technologies, Inc. of operations used to prepare and analyze biomolecules. This Application Note Santa Clara, CA describes purification of poly- and monoclonal antibodies from different media USA backgrounds constructed to mimic those encountered during antibody screening, production, and scale-up in commercial settings. Antibodies were added at differ- ent concentrations to phosphate buffered saline or supernatant media harvested from Chinese hamster ovary cell culture. Automated purifications were performed on the Agilent AssayMAP Bravo platform using specially formulated microchroma- tography cartridges containing immobilized protein A. Yields from each antibody source and dilution were not altered by the background media, or the presence of up to 10% fetal bovine serum in the media. By increasing sample throughput, the AssayMAP Bravo platform will shorten timelines for development of new antibodies for research and therapeutics. Introduction