规模猪场猪瘟免疫失败调查及免疫程序优化.pdfVIP

规模猪场猪瘟免疫失败调查及免疫程序优化 规模猪场猪瘟免疫失败调查及免疫程序优化 原霖[基金项目:北京市生猪产业技术体系创新团队 (GWZJ-2009-05) 作者简介:原霖(1982-),男,硕士,主要从事动物传染病诊断的研 究,E-mail:yuanlin_1982@163 通讯作者:顾小雪,E-mail:gxxjackie@163 顾小雪 张硕 汪葆? 胡冬梅 李晓霞 遇秀玲 翟新验 田克恭 (中国动物疫病预防控制中心,北京,100125) 摘要:本研究主要是调查规模猪场猪瘟的免疫效果,选取北京市 3 个规模猪 场,每场随机抽取 10 头仔猪分别在 25、60 和 90 日龄采血分离血清,检测 猪瘟抗体。A、B、C 三个猪场的母源抗体阳性率分别为 100%、50%、0%;二 次免疫后抗体阳性率分别为 10%、90%、90%。由于猪场A 猪瘟免疫失败, 对其免疫程序进行了优化,将首免时间推迟至 50 日龄。优化免疫程序后二 次免疫后抗体阳性率达到 70%。结果表明,母源抗体阳性率直接影响猪瘟疫 苗对仔猪的免疫效果,仔猪母源抗体降至 50%左右进行猪瘟免疫可取得比 较理想的结果。本研究为规模猪场结合仔猪母源抗体水平制定合理猪瘟免 疫方案提供了较好的参考。 关键词:规模猪场;猪瘟 ;免疫失败;免疫程序 Survey of immune failure of classical swine fever in pigs on large-scale pig farms and optimization of immunity procedure YUAN Lin,GU Xiao-xue,ZHANG Shuo,WANG Bao-yue,HU Dong-mei,LI Xiao-xia,YU Xiu-ling,ZHAI Xin-yan,TIAN Ke-gong (China Animal Disease Control Centre,Beijing,100125) Abstract:The objective of this study is to investigate immune status of classical swine fever CSF in large-scale pig farms. 3 large-scale pig farms in Beijing were chosen. Blood samples were collected from 10 piglets for each farm on 25、60 and 90-day-old individually, following to be tested antibody titers to CSFV. The positive rates of maternal antibodies to CSFV in 3 pig farms(A、B、C)were 100%,50%,0%. The positive rates of antibodies to CSFV after the second vaccination in 3 pig farms(A、B、C)reached 10%,90%,90%。In light of immune failure in pig farm A, optimization of the immunity procedure was conducted. The date of initial administration was postponed to 50-day-old. The positive rates of antibodies to CSFV after the second immunization were 70%. The results showed that the maternal antibody level directly influenced immune efficiency of CSF. The available result is to be obtained when piglets are vaccinated with a drop of 50% in positive rate of maternal antibody to CSFV. This study provides valuable references for the formulation of optimized immunity pr