HPLC—双波长切换法同时测定双参通冠胶囊中脱氢紫堇碱丹酚酸B的含量.docxVIP

HPLC—双波长切换法同时测定双参通冠胶囊中脱氢紫堇碱丹酚酸B的含量 hplc—双波长切换法同时测定双参通冠胶囊中脱氢紫堇碱、丹酚酸b的含量 　　摘要目的：建立同时测定双参通冠胶囊中脱氢紫堇碱和丹酚酸b含量的方法。方法：采用hplc-双波长切换法。色谱柱为waterssymmetryc18，以乙腈-0.05%磷酸溶液为流动相进行梯度洗脱，检测波长为286nm（丹酚酸b）、336nm（脱氢紫堇碱），流速为1.0ml/min，柱温为25℃，进样量为10μl。结果：在建立的检测条件下，脱氢紫堇碱和丹酚酸b均能达到基线分离，二者进样量线性范围分别为0.157～1.259μg、0.391～3.131μg（r均为0.9999）；精密度、重复性和稳定性试验（24h内）的rsd均小于2.00%（n=6～10）；平均加样回收率分别为101.61%、102.85%（rsd分别为3.59%、2.85%，n=6）。结论：建立的hplc-双波长切换法可同时测定双参通冠胶囊中脱氢紫堇碱和丹酚酸b含量，且方法操作简便、快速，可用于双参通冠胶囊的质量控制。 　　关键词双参通冠胶囊；hplc-双波长切换法；含量测定；丹酚酸b；脱氢紫堇碱 　　simultaneousdeterminationofdehydrocorydineandsalvianolicacidbinshuangshentongguancapsulesbyhplc-dualwavelengthswitchingmethod 　　sunjin，zhangpeng，pengqing，mengshuo，renjunguo，liujianxun（instituteofbasicmedicalsciences，xiyuanhospital，chinaacademyofchinesemedicalsciences，beijing100091，china） 　　abstractobjective：todevelopanmethodfordeterminingthecontentsofdehydrocorydineandsalvianolicacidbinshuangshentongguancapsulessimultaneously.methods：thehplc-dualwavelengthswitchingmethodwasused.thedeterminationwasperformedonwaterssymmetryc18columnwithmobilephaseconsistedofacetonitrile-0.05%phosphoricacidsolution（gradientelution）attheflowrateof1.0ml/min，thedetectionwavelengthwas286nm（salvianolicacidb）and336nm（dehydrocorydine）.thecolumntemperaturewasmaintainedat25℃，andsamplesizewas10μl.results：underthiscondition，dehydrocorydalineandsalvianolicacidbcouldbeseparatedinbaseline.thelinearrangeofthemwere0.157-1.259μgand0.391-3.131μg（r=0.9999）.rsdsofprecision，reproducibilityandstabilitytests（within24h）werealllowerthan2.00%（n=6-10）.theaveragerecoveryrateswere101.61%and102.85%（rsd=3.59%and2.85%，n=6）.conclusions：establishedhplc-dualwavelengthswitchingmethodcanbeusedforsimultaneousdeterminationofdehydrocorydineandsalvianolicacidbinshuangshentongguancapsules.themethodissimpleandrapid，andcanbeusedforthequalitycontrolofshuangshentongguancapsule. 　　keywordsshuangshentongguancapsules；hplc-dualwavelengthswitchingmethod；contentdetermination；salvianolicacidb；dehydrocorydine