冬虫夏草的人工繁殖与规模种植条件 药用价值的开发.docx

CordycepsAnalysis of sterols and fatty acids in natural and cultured derivatization followed with gas chromatography–mass spectrometry人工培植的虫草与自然野外生长的虫草营养成分含量的区别 Cordyceps by one-step Pharmaceutical and Biomedical Analysis CordycepsCordycepssinensisCordycepsTen free fatty acids namely lauric acid, myristic acid, pentadecanoic acid, palmitoleic acid, palmitic acid, linoleic acid, oleic acid, stearic acid, docosanoic acid and lignoceric acid and four free sterols including ergosterol, cholesterol, campesterol and β-sitosterol in natural (wild) Cordyceps sinensis, Cordyceps Cordyceps sinensis Cordyceps liangshanensis and gunnii, as well as cultured C. and militaris were first determined using pressurized liquid extraction (PLE), trimethylsilyl (TMS) derivatization and GC–MS analysis. The conditions such as the amount of reagent, temperature and time for TMS derivatization of analytes were optimized. Under the optimum conditions, all calibration curves showed good linearity within the tested ranges. The intra- and inter-day variations for 14 investigated compounds were less than 3.4% and 5.2%, respectively. The results showed that palmitic acid, linoleic acid, oleic acid, stearic Cordycepsacid and ergosterol are main components in natural and cultured which could be Cordyceps Cordycepsdiscriminated by hierarchical clustering analysis based on the contents of 14 investigated compounds or Cordyceps the 4 fatty acids, where the contents of palmitic acid and oleic acid in natural higher than those in the cultured ones. are significantly Cordyceps sinensisWe Cordyceps sinensis We sought to explore the adjunctive effects of Cordyceps sinensis (CS) in clinical renal transplantation. Materials and Methods Patients (n = 202) were divided randomly by lottery into a treatment (n = 93) and a control group (n = 109). Patients in the treatment group were treated with CS 1.0 g 3 times a day in addition to the immunosuppressive regimen given to the control group. We compared patie