NEB公司提供的保护碱基序列表.docx

NEB 公司提供的保护碱基序列表： Enzyme Oligo Sequence AccI AflIII AscI AvaI BamHI BglII BssHII BstEII BstXI ClaI Enzyme EcoRI GGTCGACC CGGTCGACCG CCGGTCGACCGG CACATGTG CCACATGTGG CCCACATGTGGG GGCGCGCC AGGCGCGCCT TTGGCGCGCCAA CCCCGGGG CCCCCGGGGG TCCCCCGGGGGA CGGATCCG CGGGATCCCG CGCGGATCCGCG CAGATCTG GAAGATCTTC GGAAGATCTTCC GGCGCGCC AGGCGCGCCT TTGGCGCGCCAA GGGT(A/T)ACCC AACTGCAGAACCAATGCATTGG AAAACTGCAGCCAATGCATTGGAA CTGCAGAACCAATGCATTGGATGCAT CATCGATG GATCGATC CCATCGATGG CCCATCGATGGG Oligo Sequence GGAATTCC CGGAATTCCG CCGGAATTCCGG HaeIII HindIII KpnI MluI NcoI NdeI NheI NotI GGGGCCCC AGCGGCCGCT TTGCGGCCGCAA CAAGCTTG CCAAGCTTGG CCCAAGCTTGGG GGGTACCC GGGGTACCCC CGGGGTACCCCG GACGCGTC CGACGCGTCG CCCATGGG CATGCCATGGCATG CCATATGG CCCATATGGG CGCCATATGGCG GGGTTTCATATGAAACCC GGAATTCCATATGGAATTCC GGGAATTCCATATGGAATTCCC GGCTAGCC CGGCTAGCCG CTAGCTAGCTAG TTGCGGCCGCAA ATTTGCGGCCGCTTTA AAATATGCGGCCGCTATAAA ATAAGAATGCGGCCGCTAAACTAT AAGGAAAAAAGCGGCCGCAAAAGGAAAA Enzyme NsiI PacI PmeI Oligo Sequence TGCATGCATGCA CCAATGCATTGGTTCTGCAGTT TTAATTAA GTTAATTAAC CCTTAATTAAGG GTTTAAAC GGTTTAAACC GGGTTTAAACCC AGCTTTGTTTAAACGGCGCGCCGG Chain Length 12 22 8 10 12 8 10 12 24 GCTGCAGC 8 TGCACTGCAGTGCA 14 PstI AACTGCAGAACCAATGCATTGG 22 AAAACTGCAGCCAATGCATTGGAA 24 CTGCAGAACCAATGCATTGGATGCAT 26 CCGATCGG 8 PvuI ATCGATCGAT 10 TCGCGATCGCGA 12 SacI CGAGCTCG 8 SacII GCCGCGGC 8 TCCCCGCGGGGA 12 GTCGACGTCAAAAGGCCATAGCGGCCGC 28 SalI ScaI SmaI GCGTCGACGTCTTGGCCATAGCGGCCGCGG ACGCGTCGACGTCGGCCATAGCGGCCGCGGAA GAGTACTC AAAAGTACTTTT CCCGGG CCCCGGGG CCCCCGGGGG TCCCCCGGGGGA Chain 30 32 8 12 6 8 10 12 % Cleavage Enzyme Oligo Sequence Length 2 hr 20 hr GACTAGT GACTAGTC 8 10 90 GGACTAGTCC 10 10 90 CGGACTAGTCCG 12 0 50 CTAGACTAGTCTAG 14 0 50 GGCATGCC 8 0 0 SphI CATGCATGCATG 12 0 25 ACATGCATGCATGT 14 10 50 StuI XbaI XhoI AAGGCCTT GAAGGCCTTC AAAAGGCCTTTT CTCTAGAG8 CTCTAGAG 8 0 0 GCTCTAGAGC 10 90 90 TGCTCTAGAGCA 12 75 90 CTAGTCTAGACTAG 14 75 90 8 90 10 90 12 90 8 0 90 90 90 0 CCCTCGAGGG 10 10 25 CCGCTCGAGCGG 12 10 75 CCCCGGGG 8