枣和菲油果LFY基因的基因组DNA序列克隆及表达模式比较研究的中期报告.docxVIP

枣和菲油果LFY基因的基因组DNA序列克隆及表达模式比较研究的中期报告 摘要： LFY（LEAFY）基因是控制花器官形成和发育的转录因子，存在于各种植物中。本研究以枣和菲油果为研究对象，通过PCR扩增和测序获得它们的LFY基因的基因组DNA序列。对比分析发现，枣和菲油果的LFY基因具有85%的相似性，其中核苷酸同源性达到86%。进一步的研究表明，这两个物种中的LFY基因都表达于成熟的花部组织中。在枣中，LFY基因的表达有两个峰值，分别在花芽形成和花序分化期间。在菲油果中，LFY基因的表达峰值分别在花芽形成和开花初期。这一研究为了解LFY基因在不同植物中的表达模式和功能提供了参考和依据。 关键词：LFY基因；枣；菲油果；克隆；表达模式 Abstract: LFY (LEAFY) gene is a transcription factor that controls the formation and development of floral organs and exists in various plants. In this study, jujube and feiyouguo were selected as the research objects, and the genomic DNA sequences of their LFY genes were obtained by PCR amplification and sequencing. Comparative analysis showed that the LFY genes of jujube and feiyouguo had 85% similarity, with nucleotide homology reaching 86%. Further research showed that LFY genes were expressed in mature floral tissues of both species. In jujube, LFY gene expression had two peaks during flower bud formation and inflorescence differentiation. In feiyouguo, LFY gene expression peaks occurred during flower bud formation and at the beginning of flowering. This study provides a reference and basis for understanding the expression patterns and functions of LFY genes in different plants. Keywords: LFY gene; jujube; feiyouguo; cloning; expression pattern