反胶束法同时提取玉米胚芽油脂和蛋白质的研究的中期报告.docxVIP

反胶束法同时提取玉米胚芽油脂和蛋白质的研究的中期报告 摘要： 玉米胚芽含有丰富的营养成分和生物活性物质，玉米胚芽油脂和蛋白质是其中的主要组分。目前，提取玉米胚芽油脂和蛋白质的方法较多，但存在着信号分离、低提取率等问题。本研究采用反胶束法同时提取玉米胚芽油脂和蛋白质，以进一步提高提取效率和分离纯度。 采用不同的萃取剂浓度、pH、离子强度、时间等参数进行反胶束提取实验，研究玉米胚芽油脂和蛋白质的提取量及其纯度。结果显示，反胶束法能高效地提取玉米胚芽油脂和蛋白质，最佳提取条件为：正丁醇质量浓度为0.2mol/L，pH值为8.0，NaCl浓度为0.2mol/L，反应时间为1h。 同时，进一步分离玉米胚芽油脂和蛋白质，得到了高纯度的玉米胚芽油脂和蛋白质。该研究为玉米胚芽食品加工提取高纯度油脂和蛋白质提供了一种新方法。 关键词：反胶束法；玉米胚芽油脂；玉米胚芽蛋白质；提取 Abstract: Maize germ contains rich nutrients and bioactive substances, and maize germ oil and protein are its main components. At present, there are many methods for extracting maize germ oil and protein, but there are problems such as signal separation and low extraction rate. In this study, reverse micelle method was used to simultaneously extract maize germ oil and protein to further improve the extraction efficiency and separation purity. Different parameters such as extractant concentration, pH, ion strength, and time were used in the reverse micelle extraction experiment to study the extraction amount and purity of maize germ oil and protein. The results showed that reverse micelle method could efficiently extract maize germ oil and protein, and the best extraction conditions were: n-butanol mass concentration was 0.2mol/L, pH was 8.0, NaCl concentration was 0.2mol/L, and the reaction time was 1h. At the same time, maize germ oil and protein were further separated, and high purity maize germ oil and protein were obtained. This study provides a new method for extracting high-purity oil and protein from maize germ for food processing. Keywords: Reverse micelle method; Maize germ oil; Maize germ protein; Extraction