活体内铁水平对巨噬细胞系转铁蛋白受体表达的调节.docxVIP

活体内铁水平对巨噬细胞系转铁蛋白受体表达的调节 路建平（上海医科大学病理学教研室，上海200032） 林举辉（日本冈山大学医学部病理学教室，日本） 【摘要】目的了解铁过剩、铁缺乏及对照组大鼠活体内铁水平对转铁蛋白受体（TER）表达调控机制和影响。方法用 免疫组化法测定Fe和TFRo结果 铁过剩或铁缺乏对大鼠肝枯否细胞、脾白髓树突状细胞TER表达无明显影响。当铁过剩 时，脾红髓巨噬细胞TFR表达减弱，十二指肠巨噬细胞TFH表达增强，而实质细胞TFR表达减弱。铁缺乏时，脾巨噬细胞TFR 襄达增强。结论巨噬细胞系细胞在不同铁水平时TFR表达的差异与其功能的差别有关。 【关键词】巨噬细胞转铁蛋白受体 The regulatory effects of iron concentration on the transferrin receptor expression of macrophages in vivo LU Jianpmg (Department of Pathology, Shanghai Medical University, Shanghai, 200032 China) Kaki Hayashi (Department of Pathology, Okayama University Medical School, Okayama, 700 Japan) mucosa.[Abstract] Iron - overloaded(Group A, n = 40), iron - deficient (Group B,几=30) of control (Group C, N = 30) Wistar rats were used. Iron - overload was produce by repeated intraperitoneal injections of ferric - nitrilotri - acetate (Fe - NTA) . Iron - deficiency was made by low iron regimen, spleen, liver and duodenal tissues of the rats were processed in immunohistochemical staining for transferrin receptor (TFR). Hie TFR staining increased in erythroblasts, hepatocytes and duodenal absorptive cells of iron 一 deficient, greatly decreased in that of :bon- ovedoaded rats. In the iron - overloaded rats, TFR staining was stronger in the macrophages of duodenal mucosa. The TFR. expression 山e Kupffer cells of the liver and the white pulp interdigiting cells of the spleen showed on obvious change in both of the iron deficient or . 21 . mucosa. 国际中华微量元素杂志 1999 年 10 月第 1 卷第 1 期 International Journal of Chinese Trace Element (DCIE) 1999, Vol」No.l overload condition. Conversely, the TFR positive macrophage and staining intensity of the red pulp was significantly reduced in iron - overloaded, in decreased in iron deficient rats. We suggest that the expression of TFR in macrophages of different organs to the iron concentration relat ed with their function. [Key words] Macrophage Transferrin receptor Immunohistochemistry TFR是哺乳动物细胞的结构糖蛋白，其分子量 约19万，由两个亚单位组成。TFR广泛分布于肝、 脾、肾、十二指肠等脏器。它可与载铁运铁蛋白（Tf