α-常春藤皂苷丙烯酸树脂纳米粒的研究的开题报告.docxVIP

α-常春藤皂苷丙烯酸树脂纳米粒的研究的开题报告

Introduction/Introduction

α-hederin(αH)isatriterpenoidsaponincompoundfoundinplantssuchasivy,ginsengandtea.PreviousstudieshaveshownthatαHisapotentanticanceragentthatinducesapoptosisinvarioustypesofcancercells.However,itshightoxicityandpoorsolubilityinwaterlimititsuseinclinicalapplications.Therefore,thedevelopmentofaneffectivedeliverysystemforαHishighlydesirable.

Objectives/Objectives

TheobjectiveofthisstudyistodevelopananocarriersystembasedonacrylateresinfortheefficientdeliveryofαHtocancercells.Themaingoalsare:

1.SynthesisandcharacterizationofαH-loadedacrylateresinnanoparticles

2.Evaluationofthephysicochemicalpropertiesandstabilityofthenanoparticles

3.Assessmentofthecytotoxicityandanti-cancerefficacyofthenanoparticlesinvitro

4.InvestigationoftheunderlyingmechanismsofαH-inducedapoptosisincancercells

Methods/Methodology

αH-loadedacrylateresinnanoparticleswillbesynthesizedbyananoprecipitationmethod.Thephysicochemicalproperties,includingparticlesize,zetapotential,morphologyanddrugloadingcapacity,willbecharacterizedbydynamiclightscattering(DLS),transmissionelectronmicroscopy(TEM),Fouriertransforminfraredspectroscopy(FTIR)andhigh-performanceliquidchromatography(HPLC).Thestabilityofthenanoparticleswillbeassessedunderdifferentconditions,suchaspH,temperatureandstoragetime.

Thecytotoxicityandanti-cancereffectsofthenanoparticleswillbeevaluatedbyMTTassay,colonyformationassayandflowcytometryanalysis.TheunderlyingmechanismsofαH-inducedapoptosiswillbeinvestigatedbywesternblottingandimmunofluorescencestaining.

ExpectedResults/ExpectedResults

ThesuccessfulsynthesisofαH-loadedacrylateresinnanoparticleswithsatisfactoryphysicochemicalpropertiesandhighdrugloadingefficiencyisexpected.Thenanoparticlesareexpectedtoex