PI3kAkt信号通路在周期性张应力介导的牙周膜成纤维细胞凋亡中的作用的开题报告.docxVIP

PI3kAkt信号通路在周期性张应力介导的牙周膜成纤维细胞凋亡中的作用的开题报告

Title：TheRoleofPI3kAktSignalingPathwayinPeriodontalLigamentFibroblastApoptosisMediatedbyCyclicalTensileStress

Background：

Periodontitisisacommondiseaseindentalclinic,whichiscausedbychronicinflammationofperiodontaltissues,leadingtothedestructionofperiodontalsupportingtissuesandinevitablyresultintoothloss.Periodontalligamentfibroblasts(PDLFs)arethemaincellsinperiodontaltissuesandplayacentralroleinmaintainingthehomeostasisofperiodontaltissues.Periodontaltissuesareunderconstantmechanicalstressfromchewingandspeaking,whichmaycontributetothedevelopmentofperiodontitis.However,theunderlyingmechanismofhowmechanicalstressaffectsPDLFsandcontributestoperiodontitisremainslargelyunclear.

Objectives：

ToinvestigatetheroleofPI3kAktsignalingpathwayincyclicaltensilestress-inducedPDLFapoptosis,andtodeterminethepotentialtherapeutictargetforperiodontitis.

Methods：

CyclicaltensilestresswillbeappliedtoPDLFcellsbyusingacustom-madedevicefor12hours.TheexpressionlevelofPI3kAktsignalingpathwaymarkers,includingp-Akt,p-PI3K,Bax,andBcl-2,willbemeasuredbyWesternblotandRT-PCRaftercellswereexposedtocyclicaltensilestress.Meanwhile,theapoptosisrateofPDLFwillbedetectedbyflowcytometryandTUNELstaining.Additionally,thePDLFwillbetreatedwithPI3kAktsignalingpathwayinhibitortoexplorewhethertheinhibitionofPI3kAktcanattenuatethecyclicaltensilestress-inducedapoptosis.

ExpectedResults：

OurresearchisexpectedtorevealthemechanismofcyclicaltensilestressonPDLFsapoptosisandtofindnewtherapeutictargetsforperiodontitis.Theresultsofthisstudywillimproveourunderstandingofperiodontitisandprovidesupportfornoveltherapeuticapproachesforthepreventionandtreatmentofperiodontitis.