miR-140-5p在骨肉瘤中的表达及其对骨肉瘤细胞侵袭转移的影响.pdfVIP

miR-140-5p在骨肉瘤中的表达及其对骨肉瘤细胞侵袭转移

的影响

孙玉秀;邱月;项博文;汪忠淼;秦宝丽

【摘要】ObjectiveToexploretheexpressionofmiR-140-5pin

osteosarcomaanditsinfluenceontheinvasionandmigrationofMG-63

andU2OS.MethodsTheexpressionofmiR-140-5pinosteosarcomatissue

specimenswasdeterminedbyusingqRT-PCRassayandaninsitu

hybridizationassay.TheexpressionofmiR-140-5pinMG-63,U2OSandh

FOB1.19wasmeasuredbyqRT-PCRassay.miR-140-5pmimicswere

transfectedintoMG-63andU2OScellsandwereconfirmedbyaqRT-PCR

assaytogainamiR-140-5pover-expressedcellmodel.Transwellassays

wereexecutedtoevaluatetheinfluenceofexpressionofmiR-140-5pon

themigrationandinvasionofMG-63andU2OScells.ResultsThe

expressionofmiR-140-5pwasdown-regulatedinosteosarcomatissue

specimenscomparedwiththatinpara-tumortissuespecimens,anditwas

alsodown-regulatedinosteosarcomacelllinesMG-63andU2OS

comparedwiththatofhFOB1.19.TheexpressionofmiR-140-5pwasup-

regulatedinMG-63andU2OScellswhichweretransfectedwithmiR-140-

5pmimics,thentheabilityofinvasionandmigrationofMG-63andU2OS

cellswasdecreased.ConclusionTheexpressionofmiR-140-5pisdown-

regulatedinosteosarcomaandcelllinesMG-63andU2OS.Up-regulating

miR-140-5pcansuppresstheinvasionandmigrationofMG-63andU2OS

cells.%目的研究miR-140-5p在骨肉瘤中的表达及其对骨肉瘤细胞MG-63及

U2OS侵袭转移的影响.方法qRT-PCR及免疫原位杂交法检测miR-140-5p在骨

肉瘤组织中的表达情况;qRT-PCR检测miR-140-5p在骨肉瘤细胞MG-63、

U2OS及正常成骨细胞系hFOB1.19中的表达;在MG-63、U2OS细胞中转染

miR-140-5pmimic以上调miR-140-5p的表达,qRT-PCR确认转染成功;

Transwell实验检测不同miR-140-5p表达水平对骨肉瘤细胞MG-63、U2OS侵

袭转移的影响.结果与癌旁组织比较,miR-140-5p在骨肉瘤组织中表达降低;与正

常成骨细胞系hFOB1.19比较,miR-140-5p在骨肉瘤细胞MG-63、U2OS中

表达降低;在MG-63、U2OS中转染miR-140-5pmimic后,miR-140-5p