莫诺苷对大鼠脑缺血再灌注皮层Wnt信号通路转录因子表达的影响.pdfVIP

莫诺苷对大鼠脑缺血再灌注皮层Wnt信号通路转录因子表达

的影响

艾厚喜;孙芳玲;侯虹丽;张丽;王文

【摘要】ObjectiveTostudytheeffectsofmorronisideontheexpressionof

Wntsignaling-relatedtranscriptionfactorsneurogenin2(Ngn2),Pax6and

Tbr2intheischemicipsilateralcortex7daysaftercerebralischemia-

reperfusioninrats.Methods15maleSprague-Dawleyratswererandomly

dividedintoshamgroup(n=3),ischemiagroup(n=3),andmorroniside

groups(low,mediumandhighdosagegroups,n=3).Themiddlecerebral

arterywereoccludedfor30min,andre-perfused.Morronisidewas

administeredintragastricallyonceadayatdoseof30mg/kg,90mg/kg

and270mg/kg3hoursafteroperation.TheexpressionofNgn2,Pax6and

Tbr2intheischemicipsilateralcortexweredetectedwithWesternblotting

analysis7daysafteroperation.ResultsTheexpressionofNgn2increased

intheischemiagroupcomparedwiththeshamgroup(P0.05),andit

furtherincreasedthemorronisidegroupsofmediumandhighdosage

comparedwiththeischemiagroup(P0.01).Therewasnosignificant

differencebetweentheischemiagroupandshamgroupintheexpression

ofPax6,whileitincreasedthemorronisidegroupsofmediumandhigh

dosagecomparedwiththeischemiagroup(P0.01).Therewasno

significantdifferenceamongallthegroupsintheexpressionofTbr2.

ConclusionMorronisidecouldincreasetheexpressionofNgn2andPax6in

theischemicipsilateralcortex7daysafterischemia-reperfusioninrats,

suggestingpromotingtheneurogenesisafterischemia.%目的研究莫诺苷对

脑缺血再灌注7d患侧皮层Wnt信号通路相关转录因子神经发生素2(Ngn2)、

Pax6、Tbr2表达的影响。方法健康成年雄性Sprague-Dawley大鼠随机分为假

手术组，模型组，莫诺苷小、中、大剂量组，每组3只。线栓法制备大鼠大脑中

动脉阻塞模型，造模后3h予莫诺苷30mg/kg、90mg/kg、270mg/kg每天1

次灌胃。Westernblotting法分析术后7d皮层Ngn2、Pax6、Tbr2的表达。结

果与假手术组相比，模型组皮层Ngn2表达升高(P0.05)；与模型组相比，莫诺

苷中、高剂量组Ngn2表达明显升高(P0.01)。模型组皮层Pax6的表达与假手术

组无显著性差异，莫诺苷中、高剂量组Pax6表达升高(P0