普罗布考对肾脏缺血再灌注损伤大鼠的保护作用及机制 .pdfVIP

普罗布考对肾脏缺血再灌注损伤大鼠的保护作用及机制

杨成;任星峰;彭隽

【摘要】目的观察普罗布考对缺血再灌注大鼠肾脏氧化应激损伤的保护作用,同时

探讨其作用机制.方法雄性SD大鼠30只,随机分为假手术组(S组)、缺血再灌注组

(IR组)、普罗布考治疗组(P+IR组),每组10只.P+IR组大鼠每天用普罗布考(500

mg/kg)灌胃,S组和IR组大鼠每天用等量温开水灌胃.1周后制备肾缺血再灌注大

鼠模型:3组大鼠都摘除右肾,IR组和P+IR组用无创动脉夹夹闭左侧肾动脉,30min

后松夹恢复血流再灌注.再灌注后S组和IR组每天温开水灌胃,P+IR组每天普罗布

考灌胃,连续1周.1周后处死所有大鼠,留取血及肾组织标本,检测大鼠各项血液生化

指标,观察肾组织病理学改变.结果与IR组相比,P+IR组血尿素氮(BUN)、血肌酐

(SCr)、血脂水平明显降低(均P0.05),血清和肾组织中SOD活性明显上升、MDA

含量明显下降(均P0.05),肾组织病理损伤减轻,肾指数明显改善(P0.05).结论普

罗布考能提高缺血再灌注损伤大鼠血及肾组织中SOD活性,改善肾功能,降低血脂

水平,减轻肾组织病理损伤,发挥肾脏保护作用.%ObjectiveTheaimofthestudy

wastoinvestigatetheprotectiveeffectofprobucolonoxidativestress

injuryinratswithischemia-reperfusioninjury(IRI)anditsmechanism.

MethodsThirtymaleSprague-Dawleyratswererandomlydi-videdinto

shamoperationgroup(Sgroup),ischemia-reperfusiongroup(IRgroup),and

probucoltreatmentgroup(probucol+IRgroup,P+IRgroup).RatsintheS

andIRgroupswerefedwithwarmwatereveryday,andratsinP+IRgroup

weretreatedwithprobucol.After1week,ratmodelofrenalischemiaand

reperfusionwasestablished.Rightkidneysoftheratswerere-moved.InIR

andP+IRgroups,theleftrenalarterywasclampedwithanon-invasive

arteryclamp,andafter30minutesthebloodvesselsrestoredpatencyby

looseningtheclamp.Afterreperfusion,theSandIRgroupswereperfused

withwarmwatereveryday,andtheP+IRgroupwastreatedwithprobucol

for1week.After1week,alltheratsweresacrificedandthebloodand

kidneytissuespecimensweretaken.Thebloodbiochemicalindexesofthe

ratsweremeasured,andthepathologicalchangesofthekidneyswere

observed.ResultsThelevelsofbloodureanitrogen(BUN),serum

creatinine(SCr)andbloodlipidsinP+IRgroupweresignificantlylower

thanthoseinIRgroup(allP0.05);inP+IRgroup,thelevelofsuperoxide

dismutase(SOD)inserumandrenaltissuewasincreasedsignificantly(all

P0.05),meanwhile,thelevelsofm